Sequences from confirmation amplicon


Confirmation sequences for line 176F04 and hit At1g13160 are shown.
Line ID 176F04
Locus At1g13160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4484691
Primers used for PCR CM46 - 8474, show primer details
   
Sequence Name 05-K104123-0022-176-F04C-CM46
GenBank Accession LN488329 [GenBank]
Sequencing primer gene specific primer, CM46: 5'-CTCATCTTACGAATGGTTTGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K104123-0022-176-F04C-CM46
TTTCGCAGGGTTTTTGTCACCAAGAGTCTGAATATCCAGAAACAAAGCCAGCAAATCCTC
GATGACAAGACTCTATTCACACCACAAAGAAAACATTCTCAGAACAAAACCAATCCTTTT
TAAAGTCTCAAACTTTACACACCACAAACACAAAGTAAGAAATGACAGTTTTTTCCAATC
AATTCAGATAAAAGCACAAACCTTTCGATTCATTAGAAGGATCAGAGCTTGTGCAATATG
GTTTCGAAGCCCCGAGGGCATAGCTAAGCACGAAGTACGGAGTAAACCAGTTAATTGAGC
TGGAAACGCCGCGAGTTGCTTCGGGTAAAAGGGAGTAACGTGAGCTAGAAACATGGCACG
GTCGCCGAGGTCTTTCGAAACAGAAGGGTCAGAGCCAATGCCACCAGTGGAGGAGAAACT
GAGAGCTGCTAGTTCATGGAAGAGATCGACGGAGGTTTTGAACTGTTTGTAAATCAATTG
GAGCTCTGTTTCGTAACCTTCTGGGTCACGTTTGATTTTACCTTGAAGGGTAAGAAGACT
GACATTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCG
   
Sequence Name 21-K104124-0022-176-F04C-8409
GenBank Accession LN495635 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104124-0022-176-F04C-8409
GCCATTTACAATTGAATATATCCTGAGAATGTCAGTCTTCTTACCCTTCAAGGTAAAATC
AAACGTGACCCAGAAGGTTACGAAACAGAGCTCCAATTGATTTACAAACAGTTCAAAACC
TCCGTCGATCTCTTCCATGAACTAGCAGCTCTCAGTTTCTCCTCCACTGGTGGCATTGGC
TCTGACCCTTCTGTTTCGAAAGACCTCGGCGACCGTGCCATGTTTCTAGCTCACGTTACT
CCCTTTTACCCGAAGCAACTCGCGGCGTTTCCAGCTCAATTAACTGGTTTACTCCGTACT
TCGTGCTTAGCTATGCCCTCGGGGCTTCGAAACCATATTGCACAAGCTCTGATCCTTCTA
ATGAATCGAAAGGTTTGTGCTTTTATCTGAATTGATTGGAAAAAACTGTCATTTCTTACT
TTGTGTTTGTGGTGTGTAAAGTTTGAGACTTTAAAAAGGATTGGTTTTGTTCTGAGAATG
TTTTCTTTGTGGTGTGAATAGAGTCTTGTCATCGAGGATTTGCTGGCTTTGTTTCTGGAT
ATTCAGACTCTTGGTGACAAAAACCTGCGAAATCTTGCTTTTGGTCACATTGTTCAAACC
ATTCGAAAA

Last Updated on 06.12.2018 16:11