Sequences from confirmation amplicon


Confirmation sequences for line 177D09 and hit At5g57800 are shown.
Line ID 177D09
Locus At5g57800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23421883
Primers used for PCR PH49 - 8474, show primer details
   
Sequence Name 20-K017519-022-177-D09C-PH49
GenBank Accession LN495004 [GenBank]
Sequencing primer gene specific primer, PH49: 5'-CTACGGTTACGCTGTTATGTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K017519-022-177-D09C-PH49
ATGAGGTGTTTAGGACATTGTAACGTTGAGATATTCTCTCACAAGCTGTTCGAGATTCTT
CCAGTCTTACGATATCTCATCTATACTCCAACGTATGTCTTGTTGACGTTTACCTTACCT
TACTTGAAAAGCTTTCACATGTCTATTTCTTTTTTTGTTGCCATTTCTGGATTTAGTTAG
ATTGGGGCTTCACAGGTTTTAATGAAAAGGATCTGACTTTATTCATTGAATTAGGAAAAC
ACAAACGTGACTTTGTCATATTTTTCTTTAAGAAGCAAGTTTTGTGTTTGCTGTGAAAGA
TGTCTATAGAAAGTAATTGGTGGTTTTACATTTACTGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 21-K017519-022-177-D09C-8409
GenBank Accession LN495458 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K017519-022-177-D09C-8409
CATGTAGATTTCCCGGACATGAAGCAGTAAATGTAAAACCACCAATTACTTTCTATAGAC
ATCTTTCACAGCAAACACAAAACTTGCTTCTTAAAGAAAAATATGACAAAGTCACGTTTG
TGTTTTCCTAATTCAATGAATAAAGTCAGATCCTTTTCATTAAAACCTGTGAAGCCCCAA
TCTAACTAAATCCAGAAATGGCAACAAAAAAAGAAATAGACATGTGAAAGCTTTTCAAGT
AAGGTAAGGTAAACGTCAACAAGACATACGTTGGAGTATAGATGAGATATCGTAAGACTG
GAAGAATCTCGAACAGCTTGTGAGAGAATATCTCAACGTTACAATGTCCTAAACACCTCA
TGAAATCAAACATAACAGCGTAAACCGT

Last Updated on 06.12.2018 16:11