Sequences from confirmation amplicon


Confirmation sequences for line 177E08 and hit At2g14920 are shown.
Line ID 177E08
Locus At2g14920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6418914
Primers used for PCR KT37 - 8474, show primer details
   
Sequence Name 43-K100820-0022-177-E08C-8409
GenBank Accession LN504764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K100820-0022-177-E08C-8409
TTTACAATTGAATATATCCAGCATTTTCTTTTCATGAGGTACGCGGATTTGAAAGCGGAG
CCTCGTACTCAGGTCAAGAGACTTGCAGAGTTCTTGGATTGTCCATTCACTAAGGAAGAG
GAAGATAGCGGATCTGTAGACAAGATCTTGGAACTTTGCTCTTTAAGTAATCTAAGAAGT
GTGGAGATCAACAAAACCAGAACGTCGAGTAGAGTGGATTTCAAGAGTTATTTCCGTAAA
GGACAAGTTGGTGACTGGAAGAGTTATATGACCCCTGAAATGGTAGACAAAATTGATATG
ATTATAGAGGAAAAACTCAAAGGTTCCGGTTTGAAATTCTAAAAATTATGTGTGCACTAT
TTAATCTATTAAGAAAATGTTATATTGTCATGTTTATTGTTACTTTGATGTGTGTGATTT
TTGTTCTCTTTGATGTTCAATAAAGTTACCTTCTTTTTACTTTTGTTTCATCCGTTCATG
ACTAA
   
Sequence Name 43-K100832-0022-177-E08C-KT37
GenBank Accession LN504766 [GenBank]
Sequencing primer gene specific primer, KT37: 5'-TTTAGTCATGAACGGATGAAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K100832-0022-177-E08C-KT37
GAGAAAAAAATCACACACATCAAAGTAACAATAAACATGACAATATAACATTTTCTTAAT
AGATTAAATAGTGCACACATAATTTTTAGAATTTCAAACCGGAACCTTTGAGTTTTTCCT
CTATAATCATATCAATTTTGTCTACCATTTCAGGGGTCATATAACTCTTCCAGTCACCAA
CTTGTCCTTTACGGAAATAACTCTTGAAATCCACTCTACTCGACGTTCTGGTTTTGTTGA
TCTCCACACTTCTTAGATTACTTAAAGAGCAAAGTTCCAAGATCTTGTCTACAGATCCGC
TATCTTCCTCTTCCTTAGTGAATGGACAATCCAAGAACTCTGCAAGTCTCTTGACCTGAG
TACGAGGCTCCGCTTTCAAATCCTCGTACCTCATGAAAAGAAAATGCTTGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11