Sequences from confirmation amplicon


Confirmation sequences for line 178A08 and hit MHC9 are shown.
Line ID 178A08
Locus MHC9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7516725
Primers used for PCR AH35 - 8474, show primer details
   
Sequence Name 07-K105564-0022-178-A08C-AH35
GenBank Accession LN489268 [GenBank]
Sequencing primer gene specific primer, AH35: 5'-ACAAAGCGAAGTAATCGAAGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105564-0022-178-A08C-AH35
GAGGAATTCCAGTCATATCCGTTCCCGGAGGCTGTAACGGAGGAGGAATTTGATCCGCCG
CATTGCCGTTGAAGGTATCGGCCGTAGCCGCCGAGAGCAGAGAGGAATCCATGGAAGTGA
GTCACTTCGCCGTGAAGGAGAAGAGCACAGAGAAAGAAAGAGAGCGAGAGAGAGAATAAT
ATGGTTCGGCCCATTATGTAATTTGGGCTTTCGAATTTCATTGGCCCAAAATTGAAGGAA
TTAGACAAATGTGTTATCAACTGATTCTGACAACATTTTTTTTTTTGTTACAAACTTATA
AAACCTATAAAACCTTTTTTTTTAAAAACAAGAATTTGAACTTTGCCCAAGGAAACCTTA
TTTAAAAAAAAAAAAACTTAACAAGGGCAAATAATTTTTTTTTATAAAAAGGAAATAAAA
CCCCGGGGTTTTGTCCAAAAACCCCCAGGGGTCAAAAGTCACCGCCCTTTTAAATCAAAA
AAGGGTTTTGTTGGGGGTCCCAGGCTCCTTTTCATTTGAAAAGGGCTTCAGGTCGGGGAA
ATCTCCTGGGATCACCAAGGATTAGGAGGGTCAATAGGGAAAAAAAAAAAGAGTATTTCC
CATTTTTTTTTCATTTCAAAAAGGTAAATGTCC
   
Sequence Name 55-K105565w-0022-178-A08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K105565w-0022-178-A08C-8409
TTACATTGAACGTAGCATGGAACACACAACAAAACCTTCTTATGATTTAAATGGTCGTTG
ACTTTTGACCCTTGTGGTTTTTTGGACAAAACCCTGTGTTTTTATTTCATTTCTATAAAA
AAGATTTATCTGCCTTTGTTATGTTTTCTTCTTATTAATTAAGTTATCATCGGACAAAGT
TCAATTACTAGTTATTAAAAAAAAAGGTTCTATAGTTATTATAAGTTTGTAACAAAAAAA
AAAAGGTTGTCAAAATCATTTGAAAACACTTTTGTCAAATTCCTTCATTTTGGGGCCAAG
GAATTTCAAAAGCCCAATTTACATAGGGGGCCAAACCATATTATTCTCCCCCCCGCTCTT
TTTTTTTCTCGGGGCTCTTCCCCTTCAGGGGAAAGGGACCCATTTCCAGGGATTCCTCTC
GGCTCTGGGGGGCTAGGGCGAATACTTTAAAGGGAAAGGGGGGGAATAAAATTCCCCCTC
CTTTACACCCTCGGGAAAGGGATATAACGGGAATTTCCTTCGGGGATCACCTCGGGATCA
TTTCAAACCCTCTGGATCGAAACAACTTCTTC

Last Updated on 06.12.2018 16:11