Sequences from confirmation amplicon


Confirmation sequences for line 178C02 and hit At5g26770 are shown.
Line ID 178C02
Locus At5g26770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9409176
Primers used for PCR 13we - 8474, show primer details
   
Sequence Name 74-K118393-0022-178-C02C-13we
GenBank Accession LN513147 [GenBank]
Sequencing primer gene specific primer, 13we: 5'-TTCAAAGTTGTGAGATTTGATTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118393-0022-178-C02C-13we
GACAAACCCTGATGTCGGATTCCGTCAAACGACGGTGGATCCGCTTTTGAAAGATTTGGA
TGGGAAAAAAGAGAGTTTCAGGCGGAACGTTGTGTCTATGGCAGCTGAGTTAAAGCAAGT
GAGAGGTCGTTTGGTTTCTCAAGAACAATTCTTTGTTAAAGAAAGCTTTTGTAGAAAAGT
AAGTTCCTTTTATTAATTTGTTAATCTTCTCACTGTTCCATTGTTTTTGATTCTCTGTTT
GATTTCTTACCTTTATGTTATTGTTACTGCTTTGGTAGGGTCAGAAATCTAGTTTCAATG
TTGAGAAATGATTTGTTTTTTTTGTTTCTTTTAGGAAGCAGAGAAGAAAGCTAAGAACAT
GGAAATGGAGATCTGTAAATTGCAGAAGAAGTTGGAAGATAGAAATTGTGAGCTTGTAGC
TTCAACTTCTGCTGCTGAAAAGGTTTGTTTATTCTCTCAGAACCCTTTTCATCTTATAAT
ATAGTATGACTTAAAAGACTTCCAAATTTGATATTGTTGTCTGAAAAGTTTGGGGCTTTG
AGTAGTGTTGGTACTGAAAAGTGATCATCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 26-K118392-0022-178-C02C-8409
GenBank Accession LN498130 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118392-0022-178-C02C-8409
ATTTACATTGAATATTCCTGATGATCACTTTTCAGTACCAACACTACTCATAGCCCCAAA
CTTTTCAGACAACAATATCAAATTTGGAAGTCTTTTAAGTCATACTATATTATAAGATGA
AAAGGGTTCTGAGAGAATAAACAAACCTTTTCAGCAGCAGAAGTTGAAGCTACAAGCTCA
CAATTTCTATCTTCCAACTTCTTCTGCAATTTACAGATCTCCATTTCCATGTTCTTAGCT
TTCTTCTCTGCTTCCTAAAAGAAACAAAAAAAACAAATCATTTCTCAACATTGAAACTAG
ATTTCTGACCCTACCAAAGCAGTAACAATAACATAAAGGTAAGAAATCAAACAGAGAATC
AAAAACAATGGAACAGTGAGAAGATTAACAAATTAATAAAAGGAACTTACTTTTCTACAA
AAGCTTTCTTTAACAAAGAATTGTTCTTGAGAAACCAAACGACCTCTCACTTGCTTTAAC
TCAGCTGCCATAGACACAACGTTCCGCCTGAAACTCTCTTTTTTCCCATCCAAATCTTTC
AAAAGCGGATCCACCGTCGTTTTGACGGAATCCGACATCAGGGTTTTGTCCAATTTCTCA
TAAAATTGTGAATCTGCATAAGCATCGAATCAAATC

Last Updated on 06.12.2018 16:11