Sequences from confirmation amplicon


Confirmation sequences for line 178E03 and hit At1g15570 are shown.
Line ID 178E03
Locus At1g15570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5363254
Primers used for PCR AS05 - 8474, show primer details
   
Sequence Name 02-K018321-022-178-E03C-AS05
GenBank Accession LN486830 [GenBank]
Sequencing primer gene specific primer, AS05: 5'-AAGAGAAGTGACACAGAAGCACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K018321-022-178-E03C-AS05
TCCTTTGGGTCACCAAGTTTCTTTAGAGACTATCAAAGCCTAGGAATTACCTCTGAAACA
CGCAAATTGTAGTGGATTTCAGGGGCATAGAGGCAGCACAGTAAAGGATCCTTGTCATCT
GAATCGATGTCTACAAATTTTGGGATATCCGGTACAGTTGAACTACCAATAACAGCACTT
TTCTCAGCTGTAACCATTTGCAAAAAAGATTTATAAGTCATTAAAGACCACTCCTTTATA
CAATAGGAACTAATCATATGAAAACTTGTGTTAAATGGATCACCTGTAGAAGCTGATCTC
CCAAGAGGTCTTGGAGGCAATCTAGAATTCCATATCTCAATACAGTTATCAGCTGTGTTA
TCTGTGCCTTTAGAAACAGAAAGGTTTCCTGCTGTATTTGATGCAACTTCAACTTTTGCA
TCGGTCCTGGACTGAAGATCTGTGACTTCTGAAGTAACAGAAGTTGCCAACTGGGATGTA
CTCGCCAATCCATGACCGCGTCCTTTCTTTATCTGCTTGCTGTTTTTGGCCTGAAATGGC
AAAACCCATGTTTCAGTGATGCATGTTTTACTTCACAAGATCTTTACAAAAATTCACAGG
AAACCAAACACTATGAAGAAACATAATATAGTTTTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAAT
TTTTTTTCAATT
   
Sequence Name 06-K018321-022-178-E03C-8409
GenBank Accession LN488597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018321-022-178-E03C-8409
TAGATTTCCCGGACATGAAGCCATTTAAAACTATATTATGTTTCTTTATAGTGTTTGGTT
TCCTGTGAATTTTTGTAAAGATCTTGTGAAGTAAAACATGCATCACTGAAACATGGGTTT
TGCCATTTCAGGCCAAAAACAGCAAGCAGATAAAGAAAGGACGCGGTCATGGATTGGCGA
GTACATCCCAGTTGGCAACTTCTGTTACTTCAGAAGTCACAGATCTTCAGTCCAGGACCG
ATGCAAAAGTTGAAGTTGCATCAAATACAGCAGGAAACCTTTCTGTTTCTAAAGGCACAG
ATAACACAGCTGATAACTGTATTGAGATATGGAATTCTAGATTGCCTCCAAGACCTCTTG
GGAGATCAGCTTCTACAGGTGATCCATTTAACACAAGTTTTCATATGATTAGTTCCTATT
GTATAAAGGAGTGGTCTTTAATGACTTATAAATCTTTTTTGCAAATGGTTACAGCTGAGA
AAAGTGCTGTTATTGGTAGTTCAACTGTACCGGATATCCCAAAATTTGTAGACATCGATT
CAGATGACAAGGATCCTTTACTGTGCTGCCTCTATGCCCCTGAAATCCACTACAATTTGC
GTGTTTCAGAGGTAATTCCTAGGCTTTGATAGTCTCTAAAGAAACTTGGTGAGCCAAAGG
ACTGAAAGTTTTATTGCTTGTGCTTCT

Last Updated on 06.12.2018 16:11