Sequences from confirmation amplicon


Confirmation sequences for line 178G01 and hit At1g17030 are shown.
Line ID 178G01
Locus At1g17030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5824292
Primers used for PCR 10vh - 8474, show primer details
   
Sequence Name 48-K117914-0022-178-G01C-10vh
GenBank Accession LN506630 [GenBank]
Sequencing primer gene specific primer, 10vh: 5'-CATACTTTTCCAAACGGGACTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K117914-0022-178-G01C-10vh
CCTTTCGAGCGTATCACTATTACTGCGTTCCGGGAAACGCGCGTTTTGCGGAATCTCCGT
ACGAGGTTTGTGATCCGTACAGTAATCCACAACCGCAGGAGATTCTGCAGATCTTGCCTC
ATCCAGTGTGGGAACAGTTTGGGTATCCGACAAAGAAAGGACAAGGTTGGATTGGAGATC
CAAGAACTTGGGAACTTGATGTCGGAAAACTCTCTCAGTCACTCTTCTTTTACCAGGTAT
TTTTTGTACTCCTCCTTTATATATATATATATCTGCATTTACATTATCACGAAAAGAGTA
TTAAAATTTCGAACATGTTAAAAAATGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 08-K117913-0022-178-G01C-8409
GenBank Accession LN489918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117913-0022-178-G01C-8409
ATCCTGCATTTTTTAACATCTTCGAAATTTTAATAACTCTTTTCGTGATAATGTAAATGC
AGATATATATATATATAAAGGAGGAGTACAAAAAATACCGTGGTAAAAGAAGAGTGACTG
AGAGAGTTTTCCGACATCAAGTTCCCAAGTTCTTGGATCTCCAATCCAACCTTGTCCTTT
CTTTGTCGGATACCCAAACTGTTCCCACACTGGATGAGGCAGGATCTGCAGAATCTCCCG
CGGTTGTGGATTACTGTACGGATCACAAACCTCGTACGGAGATTCCGCAAAACGCGCGTT
TCCCGGAACGCAGTAATAGTGATACGCTTCGAAGGGGAACTTCTCCGTGTCCCTCCTGTG
AACTTTAGTCCCGTTTGGAAAAGTATGAAAAAACCCGNCNTTTCCCTTTTTTTTTTTTTT
TAAAA

Last Updated on 06.12.2018 16:11