Sequences from confirmation amplicon


Confirmation sequences for line 178H04 and hit At1g73480 are shown.
Line ID 178H04
Locus At1g73480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27629386
Primers used for PCR 71pq - 8474, show primer details
   
Sequence Name 60-K115305-0022-178-H04C-71pq
GenBank Accession LN510336 [GenBank]
Sequencing primer gene specific primer, 71pq: 5'-TGTTTTATAAACAGAGGAAGCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K115305-0022-178-H04C-71pq
TTCGCTATTTTGGAGAAAACGATTGTTTTTTTTAATCGTTTCTTCATTTCTGATTCATTT
TTCTTGGTTTATGATCTGTTCAAGTAAAGGAACAATCTTAATCGCTAGAGGTAAAAATTC
AAATACAATCCCTAGATTACCCCTAAAAGGGTCATCCACAAATTACGCGAATCGAATTCA
AATTCGAAACCAATGAGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCAGTTATTAT
   
Sequence Name 25-K115304-0022-178-H04C-8409
GenBank Accession LN497581 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115304-0022-178-H04C-8409
TTGCTCATTGGTTTCGAATTTGAATTCGATTCGCGTAATTTGTGGATGACCCTTTTAGGG
GTAATCTAGGGATTGTATTTGAATTTTTACCTCTAGCGATTAAGATTGTTCCTTTACTTG
AACAGATCATAAACCAAGAAAAATGAATCAGAAATGAAGAAACGATTAAAAAAAACAATC
GTTTTTCTCCAAAATTAGCGAAATGAGAAAACTTTAGAATAGAGAATCCAAATTTAGGGC
TTCCTCTGTTTATAAAACAA

Last Updated on 06.12.2018 16:11