Sequences from confirmation amplicon


Confirmation sequences for line 179D07 and hit At5g13020 are shown.
Line ID 179D07
Locus At5g13020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4131830
Primers used for PCR 02en - 8474, show primer details
   
Sequence Name 26-K110907-0022-179-D07C-02en
GenBank Accession LN497982 [GenBank]
Sequencing primer gene specific primer, 02en: 5'-AGCTGAGGAACACTAGACTGAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K110907-0022-179-D07C-02en
TTTAATTACCTTGTTGGCTTACTTTTGATTGATTGCAGAGATTTATCAGTGATCAATTAC
ATATACAACAGACCTTATTCGCCTGATCATTTCATCAGCGTTAACCCTTGATAACAGCTC
TCTGTGTTCCTCATCAGACACTCGAAGTTCTTTTCTGAGTTCAGTGATCAAACTTTCTTT
CTCCTGAATATTTTTTTTCCAGCCGAAGGAACAAGAATAAAAAAAGGACAAAAGGTAAGC
TTGTGAAGTTACATAAACATGTACTAAACTAATGAAGAAGACAAGCAAAATAGTACCCAG
GTAATAGCATCGGACTGGGCTTTAAATGCGCGGAGTATCGAGCTATAAGCTTCTTGCTCA
ATGAGATGAATTTGAGTTTCCATTTCATTGTGCACTGAAGGCCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCA
   
Sequence Name 10-K110898-0022-179-D07C-8409
GenBank Accession LN490713 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K110898-0022-179-D07C-8409
TGAATGGAACTCAAATTCATCTCATTGAGCAAGAAGCTTATAGCTCGATACTCCGCGCAT
TTAAAGCCCAGTCCGATGCTATTACCTGGGTACTATTTTGCTTGTCTTCTTCATTAGTTT
AGTACATGTTTATGTAACTTCACAAGCTTACCTTTTGTCCTTTTTTTATTCTTGTTCCTT
CGGCTGGAAAAAAAATATTCAGGAGAAAGAAAGTTTGATCACTGAACTCAGAAAAGAACT
TCGAGTGTCTGATGAGGAACACAGAGAGCTGTTATCAAGGGTTAACGCTGATGAAATGAT
CAGGCGAATAAGGTCTGTTGTATATGTAATTGATCACTGATAAATCTCTGCAATCAATCA
AAAGTAAGCCAACAAGGTTAATTTAAATCATGTCAGGGAATGGAGAAAGGCAAACAGCCT
TCAGTCTAGTGTCCCTCAG
Primers used for PCR 03ff - 8474, show primer details
   
Sequence Name 24-K110985-0022-179-D07C-03ff
GenBank Accession LN497044 [GenBank]
Sequencing primer gene specific primer, 03ff: 5'-AGGAACACTAGACTGAAGGCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K110985-0022-179-D07C-03ff
CCTTGTTGGCTTACTTTTGATTGATTGCAGAGATTTATCAGTGATCAATTACATATACAA
CAGACCTTATTCGCCTGATCATTTCATCAGCGTTAACCCTTGATAACAGCTCTCTGTGTT
CCTCATCAGACACTCGAAGTTCTTTTCTGAGTTCAGTGATCAAACTTTCTTTCTCCTGAA
TATTTTTTTTCCAGCCGAAGGAACAAGAATAAAAAAAGGACAAAAGGTAAGCTTGTGAAG
TTACATAAACATGTACTAAACTAATGAAGAAGACAAGCAAAATAGTACCCAGGTAATAGC
ATCGGACTGGGCTTTAAATGCGCGGAGTATCGAGCTATAAGCTTCTTGCTCAATGAGATG
AATTTGAGTTTCCANNTTCATGTGCACTGAAGGCCTACATGGATCAGCAATGAGTATG
   
Sequence Name 08-K110984-0022-179-D07C-8409
GenBank Accession LN489785 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K110984-0022-179-D07C-8409
AATGGAACTCAATTCATCTCATTGAGCAAGAAGCTTATAGCTCGATACTCCGCGCATTTA
AAGCCCAGTCCGATGCTATTACCTGGGTACTATTTTGCTTGTCTTCTTCATTAGTTTAGT
ACATGTTTATGTAACTTCACAAGCTTACCTTTTGTCCTTTTTTTATTCTTGTTCCTTCGG
CTGGAAAAAAAATATTCAGGAGAAAGAAAGTTTGATCACTGAACTCAGAAAAGAACTTCG
AGTGTCTGATGAGGAACACAGAGAGCTGTTATCAAGGGTTAACGCTGATGAAATGATCAG
GCGAATAAGGTCTGTTGTATATGTAATTGATCACTGATAAATCTCTGCAATCAATCAAAA
GTAAGCCAACAAGGTTAATTTAAATCATGTCAGGGAATGGAGAAAGGCAAACAGCCTTCA
GTCTAGTGTTCC

Last Updated on 06.12.2018 16:11