Sequences from confirmation amplicon


Confirmation sequences for line 179G02 and hit At4g26630 are shown.
Line ID 179G02
Locus At4g26630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13432167
Primers used for PCR 19ew - 8474, show primer details
   
Sequence Name 29-K110985-0022-179-G02C-19ew
GenBank Accession LN499342 [GenBank]
Sequencing primer gene specific primer, 19ew: 5'-AAAACAGGATAATGACCAAACCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110985-0022-179-G02C-19ew
ACCGGGGAGGCCTTGATAGGGTTTTCAGACTTCTTCCTCTTAGAGGACGCCTTTGGACTT
GTATCACTGTCATCATCAGTCTTCTTTCGTTTGGCTGATGACCGCTTCTGTGTAATCTTC
TCTGGTGGACTGGATTTTGCAGCGACCACTGCTTTCTTGTTTCTGGCTCTCCCTGCTGAT
TCTTTCTTCCCAGCTGACAATCTAGAACCACGTTTCTTCTTTGTAGTTTCTTCTTCAGAA
TGCTCCTCAGATTCATCTTTCTCCTCGCTTTCAGAAGGCTGAGGCGCCTCATCCTCAGAT
TTATCAGGAATGCCATTTTCATTCTCCTCCTCTTTTTTCTCTTCTTTCTCTTCTGCCTTC
TCCTCCTCTTGTTTTTCTTCTTCTTCTTTCTCTTCTTCAGATTCATCATCAGAATGAGCT
AAACTCTTTTTGACAACTTTTGTTGCTTCTTCAGACTTTTTTTGGCTATATACAATTGAA
TATATCATTGCTGATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATATCCGCAGCGGTTATT
   
Sequence Name 13-K110984-0022-179-G02C-8409
GenBank Accession LN492094 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110984-0022-179-G02C-8409
GCCTTTACATTGAATATATCCTGAATCAGCAATGATATATTCAATTGTATATAGCCAAAA
AAAGTCTGAAGAAGCAACAAAAGTTGTCAAAAAGAGTTTAGCTCATTCTGATGATGAATC
TGAAGAAGAGAAAGAAGAAGAAGAAAAACAAGAGGAGGAGAAGGCAGAAGAGAAAGAAGA
GAAAAAAGAGGAGGAGAATGAAAATGGCATTCCTGATAAATCTGAGGATGAGGCGCCTCA
GCCTTCTGAAAGCGAGGAGAAAGATGAATCTGAGGAGCATTCTGAAGAAGAAACTACAAA
GAAGAAACGTGGTTCTAGATTGTCAGCTGGGAAGAAAGAATCAGCAGGGAGAGCCAGAAA
CAAGAAAGCAGTGGTCGCTGCAAAATCCAGTCCACCAGAGAAGATTACACAGAAGCGGTC
ATCAGCCAAACGAAAGAAGACTGATGATGACAGTGATACAAGTCCAAAGGCGTCCTCTAA
GAGGAAGAAGTCTGAAAACCCTATCAAGGCCTCCCCGGCTCCTTCAAAGTCTGCATCAAA
AGAGAAGCCAGGTTTGGTCATTTCCCTGTTT

Last Updated on 06.12.2018 16:11