Sequences from confirmation amplicon


Confirmation sequences for line 179G08 and hit At1g74440 are shown.
Line ID 179G08
Locus At1g74440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27979841
Primers used for PCR 11qz - 8474, show primer details
   
Sequence Name 66-K115869-0022-179-G08C-11qz
GenBank Accession LN511680 [GenBank]
Sequencing primer gene specific primer, 11qz: 5'-CACTCTCAATTAGGGTTCCTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K115869-0022-179-G08C-11qz
TTTTACCCTCACTGTGACTTATGCTGTCTTTTACATCTGTTTGGATAAGAAATCTGGTGT
TTTAGCAGCTCTGTTATGTTTCTCTTGTTGGATTGGCTCTAGCTTCCTCGCAGCTCGATT
AGGACATTCCTTGACTTTAAAGGTAAGCCCTTTGTTCTAATTACTGGAAAAGATTTCGTC
TTTATGCTTCTCCATGCATTTGAATTTTGAAGCTCAGTTTCTTCATGCTTTTGACTTGAT
AGCCATGTTGTGTCCTCTGCTCATTGTGTTCTTGTTCCTAATGTTTCTGTGGAGCTCTTG
TTTTTTTGATAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 25-K115867-0022-179-G08C-8409
GenBank Accession LN497599 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115867-0022-179-G08C-8409
TTGAATATATCCTGCTATCAAAAAAACAAGAGCTCCACAGAAACATTAGGAACAAGAACA
CAATGAGCAGAGGACACAACATGGCTATCAAGTCAAAAGCATGAAGAAACTGAGCTTCAA
AATTCAAATGCATGGAGAAGCATAAAGACGAAATCTTTTCCAGTAATTAGAACAAAGGGC
TTACCTTTAAAGTCAAGGAATGTCCTAATCGAGCTGCGAGGAAGCTAGAGCCAATCCAAC
AAGAGAAACATAACAGAGCTGCTAAAACACCAGATTTCTTATCCAAACAGATGTAAAAGA
CAGCATAAGTCACAGTGAGGGTAAAACCAATGTCCAAACGCAAAACACCATCAAATGTCA
GCGACTTGAGGAACCCATTTTGAGAGTG

Last Updated on 06.12.2018 16:11