Sequences from confirmation amplicon


Confirmation sequences for line 179G12 and hit At2g36500 are shown.
Line ID 179G12
Locus At2g36500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15318525
Primers used for PCR BL59 - 8474, show primer details
   
Sequence Name 29-K105062-0022-179-G12C-BL59
GenBank Accession LN499283 [GenBank]
Sequencing primer gene specific primer, BL59: 5'-AAAGCAATGACTTCACCATTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K105062-0022-179-G12C-BL59
ATCTTCTGAGAGCCTCAATGGCAAGTGAATCAGATGTGACAAAAATGGGATTCCTTGTCA
TGACTTTAGACACCAGAGTATGCTCTGGTCTCAACCCTTCTGCAATCACCCTCGTGGCTA
TATCTTTGTCTGTAACAATACCGGAAAGAAGAGCGCTTGAATCAGTTAACAAAACCGCAT
CAACACGCCTAGCAGCCATCCTTCGACAAGCATCAAAGACAGTGGTTCCCTCATTAATAG
TGAGAGCTTTGGATAACCGTAGCTTCTTGACTGTTCTCTCGCCATCAGAAACCGGTTGAG
CAAGAGGTGAATCAGGTTTGGAGGTGTTCTCGTTAATGCTTCCGCTTCCGCTTTCGCTCT
CTTCTGATTGAAGAACAGGTTTCTTAGAAGCTGATGATGTTCGCCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 13-K105063-0022-179-G12C-8409
GenBank Accession LN492033 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K105063-0022-179-G12C-8409
CATTTACATTGAATATATCCTGGCGAACATCATCAGCTTCTAAGAAACCTGTTCTTCAAT
CAGAAGAGAGCGAAAGCGGAAGCGGAAGCATTAACGAGAACACCTCCAAACCTGATTCAC
CTCTTGCTCAACCGGTTTCTGATGGCGAGAGAACAGTCAAGAAGCTACGGTTATCCAAAG
CTCTCACTATTAATGAGGGAACCACTGTCTTTGATGCTTGTCGAAGGATGGCTGCTAGGC
GTGTTGATGCGGTTTTGTTAACTGATTCAAGCGCTCTTCTTTCCGGTATTGTTACAGACA
AAGATATAGCCACGAGGGTGATTGCAGAAGGGTTGAGACCAGAGCATACTCTGGTGTCTA
AAGTCATGACAAGGAATCCCATTTTTGTCACATCTGATTCACTTGCCATTGAGGCTCTTC
AGAAGATGGTTCAGGGAAAGTTCAGGCACTTGCCTGTTGTAGAGAATGGTGA

Last Updated on 06.12.2018 16:11