Sequences from confirmation amplicon


Confirmation sequences for line 180C03 and hit At1g16470 are shown.
Line ID 180C03
Locus At1g16470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5623772
Primers used for PCR 12hw - 8474, show primer details
   
Sequence Name 39-K112689-0022-180-C03C-12hw
GenBank Accession LN503407 [GenBank]
Sequencing primer gene specific primer, 12hw: 5'-TCTCAAATTGCAAGTGAAAAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112689-0022-180-C03C-12hw
TTCATTCAACATATTAGGACTATGGGAGGAAGTAAAATATGACAGATGAGGTTGAATTTA
CAGTTAAATATAGCACTCACCCCGATTGAGTAAACTCTTGCATAACAGTAGCGGTTTCCC
TTACAAGTTGGGTAACAGGGATGGGTTCCTGTAACAATAAAGCTTCCTTAGGTGAAACCA
TATCACATTAGCAAGTATTTCAAGCAAATTTGAGAAAAGAGAGAGAAAGGATTACTTTGT
ACAGACGAAGATATTGCTCAGCCTGTTTCCTACTCTTCCTAACAAGAACTCGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 07-K112688-0022-180-C03C-8409
GenBank Accession LN489346 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112688-0022-180-C03C-8409
AACAGGCTGAGCAATATCTTCGTCTGTACAAAGTAATCCTTTCTCTCTCTTTTCTCAAAT
TTGCTTGAAATACTTGCTAATGTGATATGGTTTCACCTAAGGAAGCTTTATTGTTACAGG
AACCCATCCCTGTTACCCAACTTGTAAGGGAAACCGCTACTGTTATGCAAGAGTTTACTC
AATCGGGGTGAGTGCTATATTTAACTGTAAATTCAACCTCATCTGTCATATTTTTACTTC
CTCCCATAGTCCTAATATGTTTGAATTGAACTGAACTATTTCTTCAGCAAAAGTAACTGT
CGAGTGGTAGCTTTTCACTTGAAATTTGAGA

Last Updated on 06.12.2018 16:11