Sequences from confirmation amplicon


Confirmation sequences for line 182C05 and hit At4g10780 are shown.
Line ID 182C05
Locus At4g10780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6634939
Primers used for PCR BL64 - 8474, show primer details
   
Sequence Name 52-K105356-0022-182-C05C-BL64
GenBank Accession LN507815 [GenBank]
Sequencing primer gene specific primer, BL64: 5'-CAGTTTGTGTCGTCTGATATGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K105356-0022-182-C05C-BL64
TCTTAGAGTAACCAAGAGCTAAATCAACTGACAAAGAACAGATTTCGTTAGTGCTAATAC
ATTATTTATATTATTTGTTTTGATCAAACAGAGAGAGAATGGAGCTAGGAGAGGATATAC
ACCAGTTTGGTTGATGGTAAGAAATGGAGTTTAGTGGCTTCATCCTTCCATTCAACACTC
TCGATCCATTCTGTTTCTTTGTATTGTAGGACAAACTTCTTTCCAACGGTACCAGTTTTA
GAATCCAATGGAAGTTTTCTCAGCTCAGGCATGGAAAAGACATGCCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCNGTTATTA
   
Sequence Name 36-K105357-0022-182-C05C-8409
GenBank Accession LN502221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K105357-0022-182-C05C-8409
TTACAATTGGCATGTCTTTTCATGCCTGAGCTGAGAAAACTTCCATTGGATTCTAAAACT
GGTACCGTTGGAAAGAAGTTTGTCCTACAATACAAAGAAACAGAATGGATCGAGAGTGTT
GAATGGAAGGATGAAGCCACTAAACTCCATTTCTTACCATCAACCAAACTGGTGTATATC
CTCTCCTAGCTCCATTCTCTCTCTGTTTGATCAAAACAAATAATATAAATAATGTATTAG
CACTAACGAAATCTTTTCTTTGTCAGTTGATTTAGCTCTTTGGTTACTCCTAAGACAGCC
TGTAGGCCGTAGGACAAGCCGGTTTTTCAAGAACCATATCAGACGACACAAACTG

Last Updated on 06.12.2018 16:11