Sequences from confirmation amplicon


Confirmation sequences for line 182D08 and hit At2g26330 are shown.
Line ID 182D08
Locus At2g26330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11210803
Primers used for PCR SD04 - 8474, show primer details
   
Sequence Name 58-K023016-022-182-D08C-8409
GenBank Accession LN509582 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K023016-022-182-D08C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATGATTCCATTTATCTTGTTGT
TGGAAAGATCCCTTCAAATGTCATTAAATTTAATAAATGTGAGACAAGGCACATATAATG
ATGTATACTTCACAGAAAACAAGAAACACTTACAATGTATCTAAGTTACCGATACGAGAT
AGCTCAACCGGGATTGGACCTTTGATATTGTTGCTGGACAGATTACTGGTTGAACCGATA
AATCAAAGGCAACCAAAAACTCAGTAGTTAATGACTTGAAATTCGAAAAAACAAACGCTG
TGGAACTATATATTATATAAGATTAGAGAAATTCTCAGCGTCGATACTTACAGGTAAGTC
ATACTTTCTAGCTTTTGAAATGCTCGGGGTATAGTGCCACTAAACTTGTTCCCATGAACA
TTTCTACATTACATTAATGAGACACAAAATCATATAAAGCACCAATCTTGAACATTCAAG
TGAGAAGTTTTCAGAGAAAGAGATACATACAAGCTGTTTAGATTTGTGCAAGAGCTCAGA
TGATCAGGTATAGGTCCTTCCAGATCATTGTTGGCCACATTCCTATATGTACATAGTTCA
TATAAGCATAAGAACATC
   
Sequence Name 59-K023016-022-182-D08C-SD04
GenBank Accession LN509870 [GenBank]
Sequencing primer gene specific primer, SD04: 5'-ATGCTTGACATGTTTTGATGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K023016-022-182-D08C-SD04
TGCTTATATGAACTATGTACATATAGGAATGTGGCCAACAATGATCTGGAAGGACCTATA
CCTGATCATCTGAGCTCTTGCACAAATCTAAACAGCTTGTATGTATCTCTTTCTCTGAAA
ACTTCTCACTTGAATGTTCAAGATTGGTGCTTTATATGATTTTGTGTCTCATTAATGTAA
TGTAGAAATGTTCATGGGAACAAGTTTAGTGGCACTATACCCCGAGCATTTCAAAAGCTA
GAAAGTATGACTTACCTGTAAGTATCGACGCTGAGAATTTCTCTAATCTTATATAATATA
TAGTTCCACAGCGTTTGTTTTTTCGAATTTCAAGTCATTAACTACTGAGTTTTTGGTTGC
CTTTGATTTATCGGTTCAACCAGTAATCTGTCCAGCAACAATATCAAAGGTCCAATCCCG
GTTGAGCTATCTCGTATCGGTAACTTAGATACATTGTAAGTGTTTCTTGTTTTCTGTGAA
GTATACATCATTATATGTGCCTTGTCTCACATTTATTAAATTTAATGACATTTGAAGGGA
TCTTTCCAACAACAAGATAAATGGAATCATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGGAGAAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATT

Last Updated on 06.12.2018 16:11