Sequences from confirmation amplicon


Confirmation sequences for line 182H04 and hit At2g43840 are shown.
Line ID 182H04
Locus At2g43840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18157867
Primers used for PCR JK52 - 8474, show primer details
   
Sequence Name 35-K100939-0022-182-H04C-8409
GenBank Accession LN501754 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K100939-0022-182-H04C-8409
CCATTTACATTGAATCTTCTGACTTTGTTATTACGTCCCATTTTCATTTCCATGTGTCTA
TGTACTCTCCATCTTCCCAAAGATAACACAATAAAGTAAAAAGAAATAAAACTTAAAATC
CAAGCTTTTCACTTGTCACCCTTATTTAGTTTTTTTTTGGACCATTTTCTTTTCTTGCTC
TGATGAAAAGCTGTTGTCTTCTGGAAGAACCTCACAAGTCTATTAATATATGTATATTCC
CAATATCCCTTTATTCGTTCTGCGGAAAAGCAAAGATTGAGAGCGA
   
Sequence Name 35-K100946-0022-182-H04C-JK52
GenBank Accession LN501755 [GenBank]
Sequencing primer gene specific primer, JK52: 5'-TAGGAGTGACGAAAGTAGGCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K100946-0022-182-H04C-JK52
CAATCTTGATGGGAGTGTCAGCTACCATTGTTTATGTAAGAAAGATAATTGATATAGTTA
ACGGCGCAAGACTGCGTGAAGAAAGGAGCCGCAGCTAGACCAAAATCCATTGCAAGGTCA
AGCGCCCAAGGCATGAAAGAATCATAGACGATACAAGTAATAGGGTTATCAGTACTCTGG
TGTTTGCGGATGATATCAGCGACGGTTTTGGAGCCGAAGGTTTTGAAGTTTTGTAGGTAC
TCCGGGACAGAACCGGCTGATGAGAACCCTCCCTGGTCATAGCCATC

Last Updated on 06.12.2018 16:11