Sequences from confirmation amplicon


Confirmation sequences for line 183C05 and hit At5g17390 are shown.
Line ID 183C05
Locus At5g17390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5728068
Primers used for PCR 13uw - 8474, show primer details
   
Sequence Name 20-K118107-0022-183-C05C-13uw
GenBank Accession LN495406 [GenBank]
Sequencing primer gene specific primer, 13uw: 5'-TGAAGAAAACAGAAGAGCTTGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118107-0022-183-C05C-13uw
CATTACAATCAACATCTTTAATGGCACGCACGTCCTCTCTCAAAAAATTTCCTCACGTAT
ACATACGCTCAAAACATTCAAGAACCAATGCTAAGGTTCCCATTTGAGTTCTTTTTGTAA
ACAAAAACATGGCGAGATCGCTCAAGAAATCAGCCAAGTTGAGTCTAAGAAGAGTCCGGA
TCAATTCACCTTCGATTAGGTTCAAACCGGACTCGAGTTCCATAGAGAGAGACCAAAGAA
TCGAGTTTCTAGGCGAAAATGGTGATGATGCCGGATCCGGAGAAGAAGACACCAAAGAAG
CAAACAATGAAAGTGAAGAGGTTGAAGATAAGGAAGAAGAAAGAGTCAAAGTAATTGTAG
AGGAGGAAGAAGAAAAAGAACAAGATGATGCCAAGAGCTTTAACATTGGAGGCGGAGAAG
GAAAAAAAGAGGCGGTGGCAGCGGTTGAGGCGGTTGAGGAGGAGGCGGAAGGCGGGAATA
GAGTAATGGTGGTGGTTGATAAAGCGCTTGCTTCTACCGGAGCTTTAGAATGGGCTATTA
CGCATACTTTACAACCAATGAGTATGAAAAACCGTTTAGAAACCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 01-K118106-0022-183-C05C-8409
GenBank Accession LN486770 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118106-0022-183-C05C-8409
ATTGGTTTCTAACGGTTTTTCATACTCATTGGTTGTAAAGTCTGTATAATAGCCCATTCT
AAAGCTCCGGTAGAAGCAAGCGCTTTATCAACCACCACCATTACTCTATTCCCGCCTTCC
GCCTCCTCCTCAACCGCCTCAACCGCTGCCACCGCCTCTTTTTTTCCTTCTCCGCCTCCA
ATGTTAAAGCTCTTGGCATCATCTTGTTCTTTTTCTTCTTCCTCCTCTACAATTACTTTG
ACTCTTTCTTCTTCCTTATCTTCAACCTCTTCACTTTCATTGTTTGCTTCTTTGGTGTCT
TCTTCTCCGGATCCGGCATCATCACCATTTTCGCCTAGAAACTCGATTCTTTGGTCTCTC
TCTATGGAACTCGAGTCCGGTTTGAACCTAATCGAAGGTGAATTGATCCGGACTCTTCTT
AGACTCAACTTGGCTGATTTCTTGAGCGATCTCGCCATGTTTTTGTTTACAAAAAGAACT
CAAATGGGAACCTTAGCATTGGTTCTTGAATGTTTTGAGCGTATGTATACGTGAGGAAAT
TTTTTGAGAGAGGACGTGCGTGCCATTAAAGATGTTTGATTGTTAATGGTTCCACACGTT
GTTGTTTTTTTCTTTTTGTCAAGCTCTTCTGTTTTTCTTCA

Last Updated on 06.12.2018 16:11