Sequences from confirmation amplicon


Confirmation sequences for line 183C07 and hit At1g66480 are shown.
Line ID 183C07
Locus At1g66480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24806285
Primers used for PCR KA28 - 8474, show primer details
   
Sequence Name 22-K105305-0022-183-C07C-KA28
GenBank Accession LN496108 [GenBank]
Sequencing primer gene specific primer, KA28: 5'-CTAGAACCATGTTTTTGAGAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K105305-0022-183-C07C-KA28
GTTATTAGTATGTTCTACTTCTCAAGTTGAAGAGATTAGAGATGTATGGGTCTCGGTCCG
AAACTTCTGAGATTATAATGAATTATTATTAATAATTTGTGTCAATCATCATTTGTCGGA
ATTACCGAAATATCATCCCGGAATTGCGCAAATAGTTTAAATTTTTAATGTTCCTAAATT
GATGTAGAAGGCAAGAAACGGAAGTTAATTCGTTAGGCATTGCTTTGGCGTTGGATTAGA
GTTATAAAATTATTATTGTAAGCCACTAATAAAGAAAAATGGCTACCAATATGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCG
   
Sequence Name 06-K105306-0022-183-C07C-8409
GenBank Accession LN488806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K105306-0022-183-C07C-8409
CATTTACAATTGAATATATCCATATTGGTAGCCATTTTTCTTTATTAGTGGCTTACAATA
ATAATTTTATAACTCTAATCCAACGCCAAAGCAATGCCTAACGAATTAACTTCCGTTTCT
TGCCTTCTACATCAATTTAGGAACATTAAAAATTTAAACTATTTGCGCAATTCCGGGATG
ATATTTCGGTAATTCCGACAAATGATGATTGACACAAATTATTAATAATAATTCATTATA
ATCTCAGAAGTTTCGGACCGAGACCCATACATCTCTAATCTCTTCAACTTGAGAAGTAGA
ACATACTAAATTAACCCGTGTTCAAACTCAGAATCTGACCCGGCTTCTCAAAAACAGAGT
TCTA
Primers used for PCR AJ21 - 8474, show primer details
   
Sequence Name 55-K118809-0022-183-C07C-AJ21
GenBank Accession LN508950 [GenBank]
Sequencing primer gene specific primer, AJ21: 5'-CGATAAATCATTTGATGATCGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118809-0022-183-C07C-AJ21
CATTAATCTTTTTTTCCCCGAGATATTACAAAAACTAATAAACATGTGNTTTCTAAGCAT
ATCCTTCGACAATGTTATTAAGACAAAGATGTATACAATTTCATGAACATGTGAATTCAT
TTACGAATTCCTTTGTAAATCCATTTATAAGTCCACTCCTACTCCGATAAAAGTTTGACT
ATAGTAACATGCATATCATGTAAATGTGTGTTTATGTAAATTTGTTTTGTATATATTTAC
TTACGTCATACTCACGTATGTAGATGACAAAAATATACCCTAGTGGGCTTAATTAACTAA
TAATTAATTGTAATAATTATATACATGGCTTCATGAAAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 23-K118808-0022-183-C07C-8409
GenBank Accession LN496770 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118808-0022-183-C07C-8409
CCATTTTTCATGAAGCCATGTATATAATTATTACAATTAATTATTAGTTAATTAAGCCCA
CTAGGGTATATTTTTGTCATCTACATACGTGAGTATGACGTAAGTAAATATATACAAAAC
AAATTTACATAAACACACATTTACATGATATGCATGTTACTATAGTCAAACTTTTATCGG
AGTAGGAGTGGACTTATAAATGGATTTACAAAGGAATTCGTAAATGAATTCACATGTTCA
TGAAATTGTATACATCTTTGTCTTAATAACATTGTCGAAGGATATGCTTAGAAACACATG
TTTATTAGTTTTTGTAATATCTCGGGGGAAAAAAAGATTAATGCATTATCATATCTTACC
AATGATCAAGATCCTCTCGATCATCAATGAATTTATC

Last Updated on 06.12.2018 16:11