Sequences from confirmation amplicon


Confirmation sequences for line 183E08 and hit At5g05170 are shown.
Line ID 183E08
Locus At5g05170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1530939
Primers used for PCR AC44 - 8474, show primer details
   
Sequence Name 53-K027911-022-183-E08C-8409
GenBank Accession LN508035 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K027911-022-183-E08C-8409
ATGGAGAGAAAGAGAGACAGAAACCATATACTTGCAATGTTACTAATCTGCAAAAGAGGA
ACAATAGAACCAGCTGATCAGGATTTGGAACCGTAAATAGATGACAAACACAACATGGTT
TCAGTTAAACAGAACGTAATTAAGGTTTGTTCTAGAGATAGAGATAGAGATAGAGATAGA
TAGACAGAGAGAGGTGTCAAACCTGAGGAATAATAAACTGGTTGGTGAAGAGACAAACGG
CTGGCAATGTACAATACATGAGAAGAGGAATGGAGGTGATAGGGTAGATGGTGGTGTTCA
CATACGCAAACCTCTCAAGAAATTTTAGCCTCCCATTGTAACCATACCATATAGGACAAT
GCCGACTGAAGAGAATCTCAACTGAACCTAAAGCCCACCTCAGCACTTGGTTCAGACGAT
CTGAAAGATTGATAGGAGCAGAACCCTTGAAAGCTGGAAGCTTAGGCATGCAGTAAATGG
ATCGCCATCCACGGGCATGCATTTTGAACCCAGTCAGAATATCTTCTGTCACAGAACCAT
AGATCCATCCAATCTGAAGAAAGACAGCGGAAAATATAAACAAAGTGAATGCCAAAGTGG
ATATGTCAGAACAATGCAGATTCATGTAGGAGTTCAAATGAGATTATACCTCCATTCCCC
AATCTGAC
   
Sequence Name 54-K027911-022-183-E08C-AC44
GenBank Accession LN508360 [GenBank]
Sequencing primer gene specific primer, AC44: 5'-TTATGAGGATAAGTCAGATTGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K027911-022-183-E08C-AC44
CATGAATCTGCATTGTTCTGACATATCCACTTTGGCATTCACTTTGTTTATATTTTCCGC
TGTCTTTCTTCAGATTGGATGGATCTATGGTTCTGTGACAGAAGATATTCTGACTGGGTT
CAAAATGCATGCCCGTGGATGGCGATCCATTTACTGCATGCCTAAGCTTCCAGCTTTCAA
GGGTTCTGCTCCTATCAATCTTTCAGATCGTCTGAACCAAGTGCTGAGGTGGGCTTTAGG
TTCAGTTGAGATTCTCTTCAGTCGGCATTGTCCTATATGGTATGGTTACAATGGGAGGCT
AAAATTTCTTGAGAGGTTTGCGTATGTGAACACCACCATCTACCCTATCACCTCCATTCC
TCTTCTCATGTATTGTACATTGCCAGCCGTTTGTCTCTTCACCAACCAGTTTATTATTCC
TCAGGTTTGACACCTCTCTCTGTCTATCTATCTCTATCTCTATCTCTATCTCTAGAACAA
ACCTTAATTACGTTCTGTTTAACTGAAACCATGTTGTGTTTGTCATCTATTTACGGTTCC
AAATCCTGATCAGCTGGTTCTATTGTTCCTCTTTTGCAGATTAGTAACATTGCAAGTATA
TGGTTTCTGTCTCTCTTTCTCTCCATTTTCGCCACGGGTATACTAGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCATAAACAAATTATTCTATTC

Last Updated on 06.12.2018 16:11