Sequences from confirmation amplicon


Confirmation sequences for line 183E10 and hit At5g02450 are shown.
Line ID 183E10
Locus At5g02450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:533680
Primers used for PCR BL65 - 8474, show primer details
   
Sequence Name 32-K105062-0022-183-E10C-BL65
GenBank Accession LN500578 [GenBank]
Sequencing primer gene specific primer, BL65: 5'-GAATTGTTCGTCCGTAAAATCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K105062-0022-183-E10C-BL65
GATATAGCTTATCTGAAGAGAAATCGCATAGAAGAGTAGATCTGAGATTTTGTTCTTGAA
CTAAATATGTCACGATTATTGAAATTGGCAGAGAAAATGGTGGCGACAGGCTTATTCGTG
GGGCTAAACAAAGGACACGTTGTTACCAAACGCGAGCAACCTCCTCGCCCTAACAACAGA
AAAGGGGTAAGCTCTCTAATGTTACTTACTCTGTTTCTTCTACTTAGTCCTATTTGATTC
CATTGGTCACTTTTAGTTTAACTATCGGTGGTTGGTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 16-K105063-0022-183-E10C-8409
GenBank Accession LN493425 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K105063-0022-183-E10C-8409
ACAATTGAATATATCCTGACCAACCACCGATAGTTAAACTAAAAGTGACCAATGGAATCA
AATAGGACTAAGTAGAAGAAACAGAGTAAGTAACATTAGAGAGCTTACCCCTTTTCTGTT
GTTAGGGCGAGGAGGTTGCTCGCGTTTGGTAACAACGTGTCCTTTGTTTAGCCCCACGAA
TAAGCCTGTCGCCACCATTTTCTCTGCCAATTTCAATAATCGTGACATATTTAGTTCAAG
AACAAAATCTCAGATCTACTCTTCTATGCGATTTTCTCTTCAGATAAGCTATATCTATCT
TCTAATATTATCAGCTCAATTCCAAAACTGATTTTACGGANAAACAATT

Last Updated on 06.12.2018 16:11