Sequences from confirmation amplicon


Confirmation sequences for line 183F09 and hit At1g29760 are shown.
Line ID 183F09
Locus At1g29760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10423314
Primers used for PCR BL67 - 8474, show primer details
   
Sequence Name 20-K105305-0022-183-F09C-BL67
GenBank Accession LN495208 [GenBank]
Sequencing primer gene specific primer, BL67: 5'-CAATCGAATTTCAAGTCAGTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K105305-0022-183-F09C-BL67
TTTCTCCTGGCTTCTCCGTAACTGTTTTCTCTTCTTTTTCGATCCCTTTAGCACGATTAG
GTTTGGCAGACGCTTTTTAATGGCAAGAGTAGCAGGAATAAGTGATATGATCTTTGGTTA
TATGAATCCTTTTAGGTTAAAAGATACAAAACAAATGTTGAGTATTGTGTGCAAGTTTGG
TTGGGGTATGTTTTGGGCTGTTTATGTAGGCATTGTGTTGTTTGGCTTGTTGGTTTCGTC
GCTTATGATTGGTGGTTATGTGATTAACCGTATAGCGGATAAACCATTTGAGGTCAAGGA
GACCTTGAATTTTGATTACACCAAGAATAGTCCAGAAGTTACCAAGAATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACA
GTTAT
   
Sequence Name 04-K105306-0022-183-F09C-8409
GenBank Accession LN487913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K105306-0022-183-F09C-8409
GCCATTTACAATTGAATATATTCTTGGTAACTTCTGGACTATTCTTGGTGTAATCAAAAT
TCAAGGTCTCCTTGACCTCAAATGGTTTATCCGCTATACGGTTAATCACATAACCACCAA
TCATAAGCGACGAAACCAACAAGCCAAACAACACAATGCCTACATAAACAGCCCAAAACA
TACCCCAACCAAACTTGCACACAATACTCAACATTTGTTTTGTATCTTTTAACCTAAAAG
GATTCATATAACCAAAGATCATATCACTTATTCCTGCTACTCTTGCCATTAAAAAGCGTC
TGCCAAACCTAATCGTGCTAAAGGGATCGAAAAAGAAGAGAAAACAGTTACGGAGAAGCC
ATGGAGGAAATGTGAGTAAGCTTGTCATGAAACTGACTTGAAATTCGATTG

Last Updated on 06.12.2018 16:11