Sequences from confirmation amplicon


Confirmation sequences for line 184H01 and hit At2g23370 are shown.
Line ID 184H01
Locus At2g23370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9953141
Primers used for PCR 05um - 8474, show primer details
   
Sequence Name 27-K117356-0022-184-H01C-05um
GenBank Accession LN498572 [GenBank]
Sequencing primer gene specific primer, 05um: 5'-ACCATTGAGGCACTAGAACAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117356-0022-184-H01C-05um
CAGGCTCGACTCAACTGCTTTGCATTGTTCCTCAGAAAAAGACCGGCGGAGTTAAGAGCC
TCTGGAGAAAGCTGTTGGGGAGAAATAAGAAAGGTAAGAGCAAGAAGGTCCCTAATCCAT
TTGCCAACTAAGAAGATGGTGGCATCTGTCATCTAGACTATAATCTAATACCTTAATCCT
GTTTCTTTATATGGTCCCAAAGACCCAAACTATGCAAACTAAACTGTCATTTTCTAGATT
TTTTTGGCAGTATATATATAATCTCCACGTAGAGTATTACTCTATAAGATGATCATTCTG
CTTTATTCCACTTTTAGTCTCAGAAAGGATACAATCAGTAATCTTGACCCAATGAATCTT
TAGCAGGGTTAATGTAAATCTTCAACGTAGACACTAAATGCATAAGATCATTTGATATAA
TGTCATCTCAGGTCTCAAAAGAACAAATTGTGGCCTTTAAAAGAGACTTCTCAATCTCTG
TACAGAACAAACTTGGCGATGTCGTTAAGTCCAGCTTCCTTGTAGCACTGAGCAACCCGT
TCCAGTGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CG
   
Sequence Name 07-K117355-0022-184-H01C-8409
GenBank Accession LN489452 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117355-0022-184-H01C-8409
ATTTCCTGGAACGGGTTGCTCAGTGCTACAAGGAAGCTGGACTTAACGACATCGCCAAGT
TTGTTCTGTACAGAGATTGAGAAGTCTCTTTTAAAGGCCACAATTTGTTCTTTTGAGACC
TGAGATGACATTATATCAAATGATCTTATGCATTTAGTGTCTACGTTGAAGATTTACATT
AACCCTGCTAAAGATTCATTGGGTCAAGATTACTGATTGTATCCTTTCTGAGACTAAAAG
TGGAATAAAGCAGAATGATCATCTTATAGAGTAATACTCTACGTGGAGATTATATATATA
CTGCCAAAAAAATCTAGAAAATGACAGTTTAGTTTGCATAGTTTGGGTCTTTGGGACCAT
ATAAAGAAACAGGATTAAGGTATTAGATTATAGTCTAGATGACAGATGCCACCATCTTCT
TAGTTGGCAAATGGATTAGGGACCTTCTTGCTCTTACCTTTCTTATTTCTCCCCAACAGC
TTTCTCCAGAGGCTCTTAACTCCGCCGGTCTTTTTCTGAGGAACAATGGCAAAGCAGTTT
GAGTCGGAGCCTTTGCTTTTGTCTGAGAGAAGAATCTGTTCTAGTG

Last Updated on 06.12.2018 16:11