Sequences from confirmation amplicon


Confirmation sequences for line 185D07 and hit At3g21270 are shown.
Line ID 185D07
Locus At3g21270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7475657
Primers used for PCR MZ04 - 8474, show primer details
   
Sequence Name 55-K015909-022-185-D07C-8409
GenBank Accession LN508645 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K015909-022-185-D07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTTACAAAATGACATAAACATGATCGAAA
CTGTTCAGTGTCTACGTGATGGCTCAAGTCACCCAAAATCAGTTTGCTATAATTTACCAA
AATACCCCTCTCTTCTTTTCTTCTTCAGTTTTTCTCCGCTCTGTTCATAGCCAGATCGGT
CCATGGGTTATTATTATTATTACCCGGTTCGGTTCTCCTCAAACCCAAACCCATACCCGG
ATGATCCCAACCCGACCCGTCAAGCATGATCCCTCCTCCTCCAAGAACAAGCTGCATATT
ATTCGCCAACAACGAGCTAAAGCTTCCACCAATCTCCATACCCTTCACGTCTTGGTCACC
G
   
Sequence Name 56-K015909-022-185-D07C-MZ04
GenBank Accession LN508957 [GenBank]
Sequencing primer gene specific primer, MZ04: 5'-GGGAAGTTGATAAATGTTCAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K015909-022-185-D07C-mz04
CATAAAAAAGAGAAGATTTTTGAATAAAGGTTGAAGAAAAATGCAGGATCCAGCAGCATA
TTACCAGACGATGATGGCGAAGCAACAACAACAACAACAACCACAGTTTGCAGAGCAAGA
ACAGTTAAAGTGTCCTCGTTGTGACTCACCAAACACTAAATTCTGTTACTACAACAACTA
CAATCTCTCACAGCCTCGTCACTTTTGCAAAAGCTGTCGTCGTTACTGGACTAAAGGCGG
CGCTCTCCGTAACGTTCCCGTCGGTGGTGGTTCTCGTAAGAACGCAACCAAACGATCCAC
TTCTTCTTCTTCTTCTGCTTCCTCTCCTTCCAACAGTAGCCAAAACAAGAAGACGAAAAA
CCCGGATCCGGATCCTGATCCACGTAATTCTCAAAAACCGGATTTGGATCCGACCCGGAT
GCTTTACGGGTTTCCGATCGGTGACCAAGACGTGAAGGGTATGGAGATTGGTGGAAGCTT
TAGCTCGTTGTTGGCGAATAATATGCAGCTTGGTCTTGGAGGAGGAGGGATCATGCTTGA
CGGGTCGGGTTGGGATCATCCGGGTATGGGTTTGGGTTTGAGGAGAACCGAACCGGGTAA
TAATAATAATAACCCATGGACCGATCTGGCTATGAACAGAGCGGAGAAAAACTGAAGAAG
AAAAGAAGAGAGGGGTATTTTGGTAAATTATAGCAAACTGATTTTGGGTGACTTGAGCCA
TCACGTAGACACTGAACAGTTTCGATCATGTTTATGTCATTTTGTAA

Last Updated on 06.12.2018 16:11