Sequences from confirmation amplicon


Confirmation sequences for line 186A01 and hit At4g04450 are shown.
Line ID 186A01
Locus At4g04450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2221082
Primers used for PCR IS08 - 8474, show primer details
   
Sequence Name 03-K015957-022-186-A01C-8409
GenBank Accession LN487265 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K015957-022-186-A01C-8409
ATTTCCCGGACATTACTGTTATTGTTGTTCCCATTCTGCTGATTGTTCGAACCGTTGATA
ATCGAAGTTATGGCTGCAGCTAGAGCCGCGGCAAAGTTGGGATTGGAGGCGATTGCGGCA
GTAGCGGCGCTAACACTCTCACCAGCGTTTAGCGGCTGAGACGGCATATGTAAACCCGAA
AACTTAGACTGTTGGTTGTAGTACAAAGCCTGACCCATCATATGAGGCAAAACCGATTGG
TTCAACTCCACCAAACCAGACCGTTGAGAGAATTGCATCAGCGGGTTATTGGTTGGATTG
TTCCCGTTGGGTGACTCTGTGAGGTCTAATGTAATGGTTGGGAATGGTGCAGAGGCTGAG
ATAGTAGCCATGCTTGAGGAACACGGTATAATGG
   
Sequence Name 04-K015957-022-186-A01C-IS08
GenBank Accession LN487708 [GenBank]
Sequencing primer gene specific primer, IS08: 5'-AACCATATTACCGTGTTCCTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K015957-022-186-A01C-is08
GCTACTATCTCAGCCTCTGCACCATTCCCAACCATTACATTAGACCTCACAGAGTCACCC
AACGGGAACAATCCAACCAATAACCCGCTGATGCAATTCTCTCAACGGTCTGGTTTGGTG
GAGTTGAACCAATCGGTTTTGCCTCATATGATGGGTCAGGCTTTGTACTACAACCAACAG
TCTAAGTTTTCGGGTTTACATATGCCGTCTCAGCCGCTAAACGCTGGTGAGAGTGTTAGC
GCCGCTACTGCCGCAATCGCCTCCAATCCCAACTTTGCCGCGGCTCTAGCTGCAGCCATA
ACTTCGATTATCAACGGTTCGAACAATCAGCAGAATGGGAACAACAATAACAGTAATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11