Sequences from confirmation amplicon


Confirmation sequences for line 186D02 and hit At5g52470 are shown.
Line ID 186D02
Locus At5g52470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21296223
Primers used for PCR CK13 - 8474, show primer details
   
Sequence Name 05-K017721-022-186-D02C-CK13
GenBank Accession LN488149 [GenBank]
Sequencing primer gene specific primer, CK13: 5'-AAATATCAGTTCATTGAAATCGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K017721-022-186-D02C-CK13
ATATGATGACATTTCATTTACTATGAAATGTTTGAAGCCTTTTTATTTTACAGTTATATC
TTCAAAGTTTTTTTTTATTGCATTGCATCTTTTGCTTTTAAAAAAATCAGTACAATGAAA
TCGGCAAATGATGATTTTACTTGGATTCCCCTTCATAACATTTATGGGTGTTGCAAACTC
CAAGCTTCTGATGCTACTTTGTATCTTTTTACTAAAATTTTTTACTTTGAACATGCAAAT
CTGTTTTAATCTCAAAGTTTTTATTTGTCTTCATTTGGTATATCTTATCGATTTGGAAGA
AGTCTTTTCGGTTTCTTCTGGTTTTCAACAATTGCATTTTCAAGTCATTTAGAAGTTTTA
CTTTATTACTAAACTTGGAGACTCAATTGTTGTAGCAATATTGCTGAGCTAGCAGAGATT
GCTCCAAGAAATCTGCAATGATTCCATAAATGTTTCTATGTTTATGTTAATGTCTCTCTT
TATTCTCATTTTGGCTCTCTGATTATGTTTTATTTAATTGAATCCATCTATTTGCCTAAC
TCTTGAAGGCAAGAATCTTGGCCCTGAATGCCTCATTTTTCCTCAAAACTGGTGGACACT
TTGTTATCTACATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGGCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATG
   
Sequence Name 06-K017721-022-186-D02C-8409
GenBank Accession LN488593 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K017721-022-186-D02C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCATGTAGATAACAAAG
TGTCCACCAGTTTTGAGGAAAAATGAGGCATTCAGGGCCAAGATTCTTGCCTTCAAGAGT
TAGGCAAATAGATGGATTCAATTAAATAAAACATAAACAGAGAACCAAAATGAGAATAAA
GAGAGACATTAACATAAACATAGAAACATTTATGGAATCATTGCAGATTTCTTGGAGCAA
TCTCTGCTAGCTCAGCAATATTGCTACAACAATTGAGTCTCCAAGTTTAGTAATAAAGTA
AAACTTCTAAATGACTTGAAAATGCAATTGTTGAAAACCAGAAGAAACCGAAAAGACTTC
TTCCAAATCGATAAGATATACCAAATGAAGACAAATAAAAACTTTGAGATTAAAACAGAT
TTGCATGTTCAAAGTAAAAAATTTTAGTAAAAAGATACAAAGTAGCATCAGAAGCTTGGA
GTTTGCAACACCCATAAATGTTCTGAAGGGGAATCCAAGTAAAATCATCATCTGCCGATT
TCATTGTACTGATTTTTTTAAAAGCAAAAGATGCAATGCAATAAAAAAAAACTTTGAAGA
TATAACTGTAAAATAAAAAGGCTTCAAACATTTCATAGTAAATGAAATGTCATCATATGC
CGATTTCAAT

Last Updated on 06.12.2018 16:11