Sequences from confirmation amplicon


Confirmation sequences for line 186D03 and hit At1g44970 are shown.
Line ID 186D03
Locus At1g44970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17003000
Primers used for PCR EP18 - 8474, show primer details
   
Sequence Name 19-K102174-0022-186-D03C-8409
GenBank Accession LN494703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K102174-0022-186-D03C-8409
CATTACTGCATGTAAGGTTTGAACGAAGAAGACCTTGTTTCCCTATCAGGTGAGATTAAC
ATGTATTTATCTTTTGTTTAAGGAGTTGGCAAGGAAACCAAACCGAGACCGGTTTCTTAA
AACCGAATGATAACAACGTTTTTAACCAAACCAAAATGTTGCAGGAGGACATACGATTGG
AGTAGCGAGGTGCACAACGTTTAAACAAAGACTATACAATCAAAACGGTAATAACCAACC
AGACGAGACGCTAGAGAGGTCTTACTACTATGGTCTCAGGTCAATTTGTCCACCAACAGG
CGGTGACAACAATATTTCACCTCTAGACTTAGCCTCTCCTGCAAGATTCGACAACACATA
CTTCAAGCTCCTTCTATGGGGCAAAGGCTTATTGACATCAGATGAAGTGCTTCTCACTGG
CAATGTAGGCAAGACTGGTGCATTAGTGAAAGCTTATGCCGAAGACGAGAGACTTTTCTT
CCAACAATTCGCAAAGTCGATGGTTAACATGGGAAACATTCAGCCTCTTACTGGTTTTAA
TGGTGAGATCAGGAAGAGTTGTCATGTGATTAACTAAAGAAACTGTGTGTTTTTGCATTA
TTATTGTATTAAGGGGTGATTAAGAATAAGTCGTGTTATGTTTTTCGATATGTTAGTGAT
CTAATTAACGTCTTTGAATGAAAATGGACGGTTGGTTTTAAG
   
Sequence Name 19-K102178-0022-186-D03C-EP18
GenBank Accession LN494704 [GenBank]
Sequencing primer gene specific primer, EP18: 5'-CTTAAAACAACCGTCCATTTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K102178-0022-186-D03C-EP18
ATCGAAAACTAACACGACTTATTCTTAATCACCCCTTAATACAATAATAATGCAAAAACA
CACAGTTTCTTTAGTTAATCACATGACAACTCTTCCTGATCTCACCATTAAAACCAGTAA
GAGGCTGAATGTTTCCCATGTTAACCATCGACTTTGCGAATTGTTGGAAGAAAAGTCTCT
CGTCTTCGGCATAAGCTTTCACTAATGCACCAGTCTTGCCTACATTGCCAGTGAGAAGCA
CTTCATCTGATGTCAATAAGCCTTTGCCCCATAGAAGGAGCTTGAAGTATGTGTTGTCGA
ATCTTGCAGGAGAGGCTAAGTCTAGAGGTGAAATATTGTTGTCACCGCCTGTTGGTGGAC
AAATTGACCTGAGACCATAGTAGTAAGACCTCTCTAGCGTCTCGTCTGGTTGGTTATTAC
CGTTTTGATTGTATAGTCTTTGTTTAAACGTTGTGCACCTCGCTACTCCAATCGTATGTC
CTCCTGCAACATTTTGGTTTGGTTAAAAACGTTGTTATCATTCGGTTTTAAGAAACCGGT
CTCGGTTTGGTTTCCTTGCCAACTCCTTAAACAAAAGATAAATACATGTTAATCTCACCT
GATAGGGAAACAAGGTCTTCTTCGTTCAAACCTTTACATGCAGTAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11