Sequences from confirmation amplicon


Confirmation sequences for line 187B11 and hit At2g24550 are shown.
Line ID 187B11
Locus At2g24550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10429194
Primers used for PCR 11vh - 8474, show primer details
   
Sequence Name 49-K117914-0022-187-B11C-11vh
GenBank Accession LN506973 [GenBank]
Sequencing primer gene specific primer, 11vh: 5'-CCAAACCAAACCCAAACTTACTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K117914-0022-187-B11C-11vh
CGAATCAGTGTTCCTCNTTGGCACGAAACGGCGTCGTCTTCTTCTTCTTCCTCCTCGTTT
TCACTGCTTTCTCCGATAGACGACGAACTATCCGAAGATTCCTCCGGCGACTTATTATTA
TTATTACTCATCCTTAATCCAATCCCGATCTGTGATCCACCACGTCCTGATTCATCAGCC
AAGAACAGAGCCGTTTGAACAGCCTTATCCTGCGCCGAAACACCGCGATGATCTCTAACG
TACGCCGCCATGCCGATTCCAAACGACGAACCAACCATAACCTCCATATAAACTTTTCCG
ATTAAGATCTCTTTCTCTAGCCTGTGAATATACCCGAAAGACTCAGATGATCATAAATCG
CCGGCGATGTGCGTAGGTGAAGAGCTTTAACCAGCGCAGGTTAATGATATAGGAAGAGAG
ATATACAAAACGAGAAAAATGGCTTCAATATAGGTATAAATGGCTATTCAATCGTATTAC
CAATTGAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGC
   
Sequence Name 09-K117913-0022-187-B11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117913-0022-187-B11C-8409
ACTCGGACTATCAATTAGTTTGGGTTTGGTTTGGANAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11