Sequences from confirmation amplicon


Confirmation sequences for line 187C07 and hit At5g01040 are shown.
Line ID 187C07
Locus At5g01040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15734
Primers used for PCR KC74 - 8474, show primer details
   
Sequence Name 21-K103231-0022-187-C07C-8409
GenBank Accession LN495621 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K103231-0022-187-C07C-8409
ATTTACACAGATCCAGACGTCGTGGTGAAACCGTTATGCAAGGAGCAAATAATACCGGCA
GCTAATGGAAGTCTTCCCGGCCCAACGATAAACGTTAGAGAAGGCGACACTCTTGTGGTT
AATGTCATTAACAACTCAACTTACAATGTAACCATCCACTGGTAATTAGATAACTTATTG
ATCTCATTACTATTGTTTTTTTTCTTTATAATGGAAGTCGATTTCAATATCATTTCATCA
ACTAATTACTGACTTTTTAGATGTTAATAAAAGTTATTTCTTAATTTCACACATATATGT
ATAGGCATGGAGTTTTCCAGTTGAAGAGCGTATGGATGGACGGTGCGAATATGATAACTC
AATGTCCGATTCAACCAGGTTACAACTTCACATATCAATTTGATATCACTGGACAAGAAG
GTACATTGTTATGGCACGCACACGTCGTTAATCTACGTGCCACTCTTCACGGGGCTCTAG
TCATCCGTCCCCGATCCGGCCGTCCTTACCCTTTTCCTAAACCCT
   
Sequence Name 69-K103232-0022-187-C07C-KC74
GenBank Accession LN512179 [GenBank]
Sequencing primer gene specific primer, KC74: 5'-TAGGGTTTAGGAAAAGGGTAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K103232-0022-187-C07C-KC74
GAGTGGACGTAGATAACGACGTGTGCGTGCCATAACAATGTACCTTCTTGTCCAGTGATA
TCAAATTGATATGTGAAGTTGTAACCTGGTTGAATCGGACATTGAGTTATCATATTCGCA
CCGTCCATCCATACGCTCTTCAACTGGAAAACTCCATGCCTATACATATATGTGTGAAAT
TAAGAAATAACTTTTATTAACATCTAAAAAGTCAGTAATTAGTTGATGAAATGATATTGA
AATCGACTTCCATTATAAAGAAAAAAAACAATAGTAATGAGATCAATAAGTTATCTAATT
ACCAGTGGATGGTTACATTGTAAGTTGAGTTGTTAATGACATTAACCACAAGAGTGTCGC
CTTCTCTAACGTTTATCGTTGGGCCGGGAAGACTTCCATTAGCTGCCGGTATTATTTGCT
CCTTGCATAACGGTTTCACCACGACGTCTTGGATCTGTTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTTCACTCCCA

Last Updated on 06.12.2018 16:11