Sequences from confirmation amplicon


Confirmation sequences for line 187C10 and hit At5g59160 are shown.
Line ID 187C10
Locus At5g59160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23880891
Primers used for PCR AO02 - 8474, show primer details
   
Sequence Name 07-K018611-022-187-C10C-AO02
GenBank Accession LN489051 [GenBank]
Sequencing primer gene specific primer, AO02: 5'-ATGCAAAAATGAAACAAGTTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K018611-022-187-C10C-AO02
GAATCTGAGATCCAAGCTCTGAGCTCATATTCACAAAGTTCAAAACGAAACCCCTTCTGT
TTTACTCAATCAAATCTTAAACAAGTTGTGTAAGCAAAAGATGAACAAATGTCATAATCT
AAGTAATTTGAGAAGAAATGCATGATGAACTTTTTATAGCAATCTGGAAATCTGAAAGAC
TGAAACAGGAATTGAAACACTCATCACAAGTTAGTATAAAAGACTTGAACTACCTCTTTA
CTTCATAAGAACCGGGGCTTTCGATCAGCAGGCTTAAGAATTTGGAAAGAGCACATTAAA
CTTTCATCAACACTCATCATAGCACCAGCATTGTCAAATTCACCACAGTAGTTGGGAGCA
GAAAATATCGTAACAAGCTGTCTATCGGCGAAAAACTCATATCCATCCTCTACAACCTTT
TAGAAAAAGAAAAACACAACAGTTATATTTTTATAGAATCCACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
   
Sequence Name 08-K018611-022-187-C10C-8409
GenBank Accession LN489519 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K018611-022-187-C10C-8409
CCATGTGGATTCCATAAAAATATAACTGTTGTGTTTTTCTTTTTCTAAAAGGTTGTAGAG
GATGGATATGAGTTTTTCGCCGATAGACAGCTTGTTACGATATTTTCTGCTCCCAACTAC
TGTGGTGAATTTGACAATGCTGGTGCTATGATGAGTGTTGATGAAAGTTTAATGTGCTCT
TTCCAAATTCTTAAGCCTGCTGATCGAAAGCCCCGGTTCTTATGAAGTAAAGAGGTAGTT
CAAGTCTTTTATACTAACTTGTGATGAGTGTTTCAATTCCTGTTTCAGTCTTTCAGATTT
CCAGATTGCTATAAAAAGTTCATCATGCATTTCTTCTCAAATTACTTAGATTATGACATT
TGTTCATCTTTTGCTTACACAACTTGTTTAAGATTTGATTGAGTAAAACAGAAGGGGTTT
CGTTTTGAACTTTGTGAATATGAGCTCAGAGCTTGGATCTCAGATTCAATCACTTACAGG
AAAACTTGTTTC

Last Updated on 06.12.2018 16:11