Sequences from confirmation amplicon


Confirmation sequences for line 187E10 and hit At2g27960 are shown.
Line ID 187E10
Locus At2g27960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11911097
Primers used for PCR MJ24 - 8474, show primer details
   
Sequence Name 23-K027487-022-187-E10C-8409
GenBank Accession LN496419 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K027487-022-187-E10C-8409
CTTACATTGAAATTTACATTGTATTGATTGGTGGTGATGGTAAAAATGTTTGGAATTTCG
AAGAACGAATGGCGAGCGATAGGAGTGCAGCAAAGCCGCGGATGGGTACATTACGCGGTT
CATCGACCTGAGCCGCACATAATGCTATTCAGGAGGCCTCTTAACTATCAGCAGCAGCAG
GAGAATCAAGCTCAGAACATGCTTGTTAAGTGAATCATTATCATCACCTGAGTAAGAATG
TTATATGCAACAATTCTATGAGTATTGGTTTATGTTTCTTGTAAACATGGTTTGAATCTT
TGTGGTTATGGATGAATATGTGAGAGTTGGTTTGTTGAACAACTTCTATGTAATGTTAGT
CTTGGTTCTAATGTCATCTTCTGCTTCTTAATTACTTATTTCTCAAGACATTTGGTGGTA
GTTTAGTGTCATCCAACTATGTGTTTGATTTTTAGATATATTGGTAAAA
   
Sequence Name 24-K027487-022-187-E10C-MJ24
GenBank Accession LN496857 [GenBank]
Sequencing primer gene specific primer, MJ24: 5'-ATGCCTATCCAGTGACCATTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K027487-022-187-E10C-MJ24
TGGATGACACTAAACTACCACCAAATGTCTTGAGAAATAAGTAATTAAGAAGCAGAAGAT
GACATTAGAACCAAGACTAACATTACATAGAAGTTGTTCAACAAACCAACTCTCACATAT
TCATCCATAACCACAAAGATTCAAACCATGTTTACAAGAAACATAAACCAATACTCATAG
AATTGTTGCATATAACATTCTTACTCAGGTGATGATAATGATTCACTTAACAAGCATGTT
CTGAGCTTGATTCTCCTGCTGCTGCTGATAGTTAAGAGGCCTCCTGAATAGCATTATGTG
CGGCTCAGGTCGATGAACCGCGTAATGTACCCATCCGCGGCTTTGCTGCACTCCTATCGC
TCGCCATTCGTTCTTCGAAATTCCAAACATTTTTACCATCACCACCAATCAATACAATTG
TAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNT
CGACGTGTCTAC

Last Updated on 06.12.2018 16:11