Sequences from confirmation amplicon


Confirmation sequences for line 187G04 and hit At4g01080 are shown.
Line ID 187G04
Locus At4g01080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:468226
Primers used for PCR DE46 - 8474, show primer details
   
Sequence Name 51-K104987-0022-187-G04C-DE46
GenBank Accession LN507495 [GenBank]
Sequencing primer gene specific primer, DE46: 5'-AGAAACTGCGATGGACTAAACCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K104987-0022-187-G04C-DE46
TTCACATCTGGTCGTCCATTCAATAGGCAGTTCTGAAAATCTTGAATGTGACGACAAGTG
ACATTTGTGTACAGAGGACCTGTTGGATCTGGTATCCAATCTCCGGTGAAAAGATCACAC
TTTGCTGATGAAACTACAAAAATGAAATCACAAATAGACATTTAATCAAATACATGGTAA
ACACAAACACTTTAAACCCTAAATTACAAGTTTTGATCGAAAGCTTCGAAATTTCCAAAA
TTAAACCCAACCCAACAATCATTGAATCTCCATATTTGGAAAGATTCTCGAATCTTTATC
AAAGATCAAATTCGAAATTGAAGGACAACGAACTGCGAAAGAAAGATTACCGTTTCCGGG
AGAAGATTGCGGCAATGTTGGGGGAATTGGAGAGACGGCGAGACTGGAGATGATGAAATA
GTAAGCAAGAGCGACGAGAGAGAAGTAAAGGAAGAATTTGACGAAGGTTCGATGGCGGTG
ATCAGTGCAGGAGGGAACAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCGGCTTCTTC
AAAGGTTTCTACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATT
   
Sequence Name 35-K104988-0022-187-G04C-8409
GenBank Accession LN501826 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104988-0022-187-G04C-8409
CATTTACAATTGAATATGTAGAAACCTTTGAAGAAGCCGCTTCTTCTTCTTCTTCTTCTT
CTTCTTCTTCTGTTCCCTCCTGCACTGATCACCGCCATCGAACCTTCGTCAAATTCTTCC
TTTACTTCTCTCTCGTCGCTCTTGCTTACTATTTCATCATCTCCAGTCTCGCCGTCTCTC
CAATTCCCCCAACATTGCCGCAATCTTCTCCCGGAAACGGTAATCTTTCTTTCGCAGTTC
GTTGTCCTTCAATTTCGAATTTGATCTTTGATAAAGATTCGAGAATCTTTCCAAATATGG
AGATTCAATGATTGTTGGGTTGGGTTTAATTTTGGAAATTTCGAAGCTTTCGATCAAAAC
TTGTAATTTAGGGTTTAAAGTGTTTGTGTTTACCATGTATTTGATTAAATGTCTATTTGT
GATTTCATTTTTGTAGTTTCATCAGCAAAGTGTGATCTTTTCACCGGAGATTGGATACCA
GATCCAACAGGTCCTCTGTACACAAATGTCACTTGTCGTCACATTCAAGATTTTCAGAAC
TGCCTATTGAATGGACGACCAGATGTGAATTATCTCTTCTGGAGATGGAAGCCTCGTGAT
TGTGATCTTCCTAGGTTT

Last Updated on 06.12.2018 16:11