Sequences from confirmation amplicon


Confirmation sequences for line 188A06 and hit At4g02660 are shown.
Line ID 188A06
Locus At4g02660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1167735
Primers used for PCR 15pv - 8474, show primer details
   
Sequence Name 42-K115234-0022-188-A06C-15pv
GenBank Accession LN504575 [GenBank]
Sequencing primer gene specific primer, 15pv: 5'-TTTCCGGATATTTTCTGTTAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115234-0022-188-A06C-15pv
CTTATTTTCAGAAGATGGTATTCTGACCCTTGCCACTAGCAATTCAAATTCTTTGTCATT
TTCTGGATTAGAAACTGAGGAGGGCAAGTGGCATCACCTCGCTGTTGTCCATAGTAAGCC
GAATGCTCTTGCTGGACTCTTTCAGGCTAGCGTTGCTTATGTTTACATTGATGGAAAACT
AAGGCACATGGGTAAACTGGGGTATTCTCCTTCACCTGTTGGAAAATCTTTGCAAGTAAT
AATTGGAACTTCAGCCACTTGTGCTAGGGTTAGCGATTTGACATGGAAAATACGCTCATG
TTATCTTTTTGAGGAGGGGCTCACGTCAGGCTGCATTGGTTTCATGTACATTCTTGGTAG
AGGGTATACAGGTCTTTTTCCGGGAAATGTAAATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCC
   
Sequence Name 18-K115233-0022-188-A06C-8409
GenBank Accession LN494430 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115233-0022-188-A06C-8409
CCTCTACCAAGAATGTACATGAAACCAATGCAGCCTGACGTGAGCCCCTCCTCAAAAAGA
TAACATGAGCGTATTTTCCATGTCAAATCGCTAACCCTAGCACAAGTGGCTGAAGTTCCA
ATTATTACTTGCAAAGATTTTCCAACAGGTGAAGGAGAATACCCCAGTTTACCCATGTGC
CTTAGTTTTCCATCAATGTAAACATAAGCAACGCTAGCCTGAAAGAGTCCAGCAAGAGCA
TTCGGCTTACTATGGACAACAGCGAGGTGATGCCACTTGCCCTCCTCAGTTTCTAATCCA
GAAAATGACAAAGAATTTGAATTGCTAGTGGCAAGGGTCAGAATACCATCTTCTTGAAAA
TAAAGTTCTGCATAGGATGGACTTCCATTACTTATAGCATTAACAGAAAATATCCGGAA

Last Updated on 06.12.2018 16:11