Sequences from confirmation amplicon


Confirmation sequences for line 188B06 and hit At3g15590 are shown.
Line ID 188B06
Locus At3g15590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5276434
Primers used for PCR KT22 - 8474, show primer details
   
Sequence Name 57-K100815-0022-188-B06C-8409
GenBank Accession LN509349 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K100815-0022-188-B06C-8409
TTACAATTGAATATATCCTGAACCTTTACTATTGATTAAAAAATGATAGGTAGCACGACT
CGGCTTGATGTTTTCTCTCTCCATTAGCAACAAAACATCACTTATCTTCTTTCTATCATG
CATACTGTAGAGGAGAAGCAGCTGGTTGCAAGCAAACACACTTGTTGGAAATTTTAGCTC
TTTCATTTTGTTGAAAATGTCTTCAGCTTTATTTACATGATGCTTAAGTACACAATTTGC
CAGCAGGGTTCTGTATACTACTTCTCCCCTAGAAGATTCTGGAATATCTTTCAAAAAGAT
TTCAGCTTTTTGTAAACTATGTACCTTGGCCACCAAATCCAACTGAGAAGCATAATTTGC
CTCTGTGAACTCAAACTGCGTATTAGCTCCCAACCACTCCCACAACTGCAAATGCCAAAG
CCCAAGCAAAGACATACACATTAAGAGGGTGATAGAGACAGAACAATAAAAGATTTGGCA
AAGTGTAAAATGAGAACATATTTACCTGCAAACACATAGCANAGGATTTGCGTTTACGCA
GATNGTGTATAGCAAGAGTAACCTCAGCTTGACTCANATCTNTTCCTTCTNTGACCCATT
TTT
   
Sequence Name 57-K100827-0022-188-B06C-KT22
GenBank Accession LN509351 [GenBank]
Sequencing primer gene specific primer, KT22: 5'-GAAACATGTTCTTGAAAAATGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K100827-0022-188-B06C-KT22
TCTTGCTATACACAATCTGCGTAAACGCAAATCCTATGCTATGTGTTTGCAGGTAAATAT
GTTCTCATTTTACACTTTGCCAAATCTTTTATTGTTCTGTCTCTATCACCCTCTTAATGT
GTATGTCTTTGCTTGGGCTTTGGCATTTGCAGTTGTGGGAGTGGTTGGGAGCTAATACGC
AGTTTGAGTTCACAGAGGCAAATTATGCTTCTCAGTTGGATTTGGTGGCCAAGGTACATA
GTTTACAAAAAGCTGAAATCTTTTTGAAAGATATTCCAGAATCTTCTAGGGGAGAAGTAG
TATACAGAACCCTGCTGGCAAATTGTGTACTTAAGCATCATGTAAATAAAGCTGAAGACA
TTTTCAACAAAATGAAAGAGCTAAAATTTCCNACAAGTGTGTTTGCTTGCAACCAGCTGC
TTCTCCTCTACNGTATGCATGATAGAAAGAAGATNAGTGATGTTTTGTTGCTNATGGAGA
GAGAAAACATCANGCCGAGTCGTGCTACCTATCANTTTTTAATCAATAGTANAGGTTCAG
GATATATTCAATTGTAAATGGCTTCATGTCCNGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATG
TAGATT

Last Updated on 06.12.2018 16:11