Sequences from confirmation amplicon


Confirmation sequences for line 188C07 and hit At4g13930 are shown.
Line ID 188C07
Locus At4g13930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8048557
Primers used for PCR PB34 - 8474, show primer details
   
Sequence Name 93-K102437-0022-188-C07C-8409
GenBank Accession LN515247 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K102437-0022-188-C07C-8409
ATTTACAATTGGACTGAAGCCATTTATCTGAGATATATCATAGGGCGAAGATCCCAGAGA
ACAAGGTGGTTCTCAGTTCCATTGGTCACAATCTGGTAACCCTTGCTCATTAGGTAGTTT
CCAAGGGCAACAGCATTGGCTTTCACTTGTTTTGCGTAGACCTTGAACCCAGGAGTATTA
GCCTGCTTCAACGCAACAGCTAGAGCACCAATCTGGTGATTGTGAGGACCACCTTGAAGC
GCAGGGAATACAGCAAAGTTGATTTTGTCCTCAAAATCATAGACTGCACCCTCGGGTTGA
CCCTTCTTTGGTGGTTTTGGACCCTTCCTGTAGAAAATCATACC
   
Sequence Name 93-K102438-0022-188-C07C-PB34
GenBank Accession LN515248 [GenBank]
Sequencing primer gene specific primer, PB34: 5'-TGGTATGATTTTCTACAGGAAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K102438-0022-188-C07C-PB34
CCCGAGGGTGCGTCTATGATTTTGAGGACAAATCAACTTTGCTGTATTCCCTGCGCTTCA
AGGTGGTCCTCACAATCACCAGATTGGTGCTCTAGCTGTTGCGTTGAAGCAGGCTAATAC
TCCTGGGTTCAAGGTCTACGCAAAACAAGTGAAAGCCAATGCTGTTGCCCTTGGAAACTA
CCTAATGAGCAAGGGTTACCAGATTGTGACCAATGGAACTGAGAACCACCTTGTTCTCTG
GGATCTTCGCCCTATGATATATCTCAGATAAATGGCTTCATGTCCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCNAA
AATGG

Last Updated on 06.12.2018 16:11