Sequences from confirmation amplicon


Confirmation sequences for line 188H02 and hit At1g29390 are shown.
Line ID 188H02
Locus At1g29390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10287655
Primers used for PCR IN30 - 8474, show primer details
   
Sequence Name 39-K033542-022-188-H02C-8409
GenBank Accession LN503263 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K033542-022-188-H02C-8409
TTACAATTGAATAATCCTGGTTATCGGCTGATCGTCAGCGAACCGCTACGGTGTCGGCGA
GGGCTGAGAAGCGGCGAAAGAGAGGATCGAGTGTGGTCTGTTATGCTACTCCAATGTTGT
CTGTTCATAATCTACAATGGATCTCGACAATTTCTTGCGTGTATGATCTCTCACTCTCAT
TAGCTGTGAATTTTAACGCAGAATGCTGCTGAAGATATGCTAATGTATGAAGTTTCTTAC
AAGTTTAGCTTAATCGGATAATAGCAAATGATTGTACAGGGAAATAGTTTAGCTTCAATT
CATGTGTGAAAGTGATTTGTGATAGATAATTGTATAATTTCCACTGATTCATAATGTCAG
CAGCATATAAGTTCATTTCTATACTGAATGTTGTTTCTTTTCTAGTGCATTGATGTTTGC
AAGGGGTACTGGTATCCATAAGTCCTTTGTTGTTCCACTATTCGCACTA
   
Sequence Name 39-K033542-022-188-H02C-IN30
GenBank Accession LN503264 [GenBank]
Sequencing primer gene specific primer, IN30: 5'-GTAGTGCGAATAGTGGAACAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K033542-022-188-H02C-IN30
ACATCAATGCACTAGAAAAGAAACAACATTCAGTATAGAAATGAACTTATATGCTGCTGA
CATTATGAATCAGTGGAAATTATACAATTATCTATCACAAATCACTTTCACACATGAATT
GAAGCTAAACTATTTCCCTGTACAATCATTTGCTATTATCCGATTAAGCTAAACTTGTAA
GAAACTTCATACATTAGCATATCTTCAGCAGCATTCTGCGTTAAAATTCACAGCTAATGA
GAGTGAGAGATCATACACGCAAGAAATTGTCGAGATCCATTGTAGATTATGAACAGACAA
CATTGGAGTAGCATAACAGACCACACTCGATCCTCTCTTTCGCCGCTTCTCAGCCCTCGC
CGACACCGTAGCGGTTCGCTGACGATCAGCCGATAACCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11