Sequences from confirmation amplicon


Confirmation sequences for line 188H04 and hit At4g27630 are shown.
Line ID 188H04
Locus At4g27630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13793289
Primers used for PCR 06wu - 8474, show primer details
   
Sequence Name 32-K118761-0022-188-H04C-06wu
GenBank Accession LN500772 [GenBank]
Sequencing primer gene specific primer, 06wu: 5'-TAAACATGATAATGCCAGAGCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118761-0022-188-H04C-06wu
CTCAAACAGTGGATACATACAAGATCGTATAAAGACGAATGGGAGAAATTTAAAAGCTGA
AATCAGAACTCACCTTCATCAGATTCGTCAAGAATCCCCTCACTGATATCACGATCAACA
TCCCAATAAATAACAGGGAAATATACTGAAAATAATAAACAAAGAGAGAGCATCAGACCT
ACATAAGGTAAGTTGAGAACCATATAAGCATCAAGATAAAAAGCCATCTAGTGTAACAAG
GTCTAAGAAATGAGAAAATTCACATCCCATCAAAGGCCCTATGAAACTAGAGTTATTAAT
TGAGAAACAATAATCCTATAGCTTTTCATCTGGACGATATGTTGTAAGGAGAAAGAATAT
TAACAAGCCTGCTTGCAATTCTTTGAATCTACTACTCCATACAACAAAAATCCAAAGTCA
CACAAACCTGTGAGAGCAATGCTGCATCTACTCCTATATCAAAGAACTGCAAGAATATGC
TGATCATCATCGTGACAGGATCTTTCGTGCCCGCCTGTAAACAAGCTAATCACTATTCCA
TGTCGTTTACAATAACAACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATATCCGCA
   
Sequence Name 07-K118760-0022-188-H04C-8409
GenBank Accession LN489486 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118760-0022-188-H04C-8409
TGTTATTGTAACGACATGGAATAGTGATTAGCTTGTTTACAGGCGGGCACGAAAGATCCT
GTCACGATGATGATCAGCATATTCTTGCAGTTCTTTGATATAGGAGTAGATGCAGCATTG
CTCTCACAGGTTTGTGTGACTTTGGATTTTTGTTGTATGGAGTAGTAGATTCAAAGAATT
GCAAGCAGGCTTGTTAATATTCTTTCTCCTTACAACATATCGTCCAGATGAAAAGCTATA
GGATTATTGTTTCTCAATTAATAACTCTAGTTTCATAGGGCCTTTGATGGGATGTGAATT
TTCTCATTTCTTAGACCTTGTTACACTAGATGGCTTTTTATCTTGATGCTTATATGGTTC
TCAACTTACCTTATGTAGGTCTGATGCTCTCTCTTTGTTTATTATTTTCAGTATATTTCC
CTGTTATTTATTGGGATGTTGATCGTGATATCAGTGAGGGGATTCTTGACGAATCTGATG
AAGGTGAGTTCTGATTTCAGCTTTTAAATTTCTCCCATTCGTCTTTATACGATCTTGTAT
GTTATCCACTTGTTTTGGAGCTATATAGTGTCAATGGAAAACTTGCTCTGGCATTACCAT
GTTT

Last Updated on 06.12.2018 16:11