Sequences from confirmation amplicon


Confirmation sequences for line 189B03 and hit At5g05270 are shown.
Line ID 189B03
Locus At5g05270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1564227
Primers used for PCR BL72 - 8474, show primer details
   
Sequence Name 29-K105138-0022-189-B03C-BL72
GenBank Accession LN499284 [GenBank]
Sequencing primer gene specific primer, BL72: 5'-TAGGACATTGATTGTTTTGTACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K105138-0022-189-B03C-BL72
TCTTCAACAATTCTCATTAGTATAGTTATACTGTATAACGCAAAAAACAGAGTTCCTCTG
TTCCACAAGAATTTGATGCTTATGTTTATGGATAACTAAACATACTTTCTTTCTAAAAAT
ATATGAAAGTTGATATTTTCTTACCTCGCAAATGCCATCTTTGGCTGAGAAATGGTAAGT
GATAACGGAGTTAGCTTTGAAGTACTTGGACTGGAAAAAGCCAACGACCTTCTCGAGCTC
AGTTTCTTCTTCTTCCTCGTACTTATCCTCCTCAGCCAAACGATCTCTCACCGTATTCTC
TAGCTGCACTCCGTACTGAGCTCCTTTTATCTCCTTTATCAATTGTAGATTTTCAGTGTA
GTCAATTAGAGGGTCAGGATATGTGTTCTCAAGTATTCTTCTCTCTGATGCCCTGATATG
TGTTCAGGGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCC
   
Sequence Name 13-K105139-0022-189-B03C-8409
GenBank Accession LN492034 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K105139-0022-189-B03C-8409
CATTTACATTGAATATATCCTGACCCTGAACACATATCAGGGCATCAGAGAGAAGAATAC
TTGAGAACACATATCCTGACCCTCTAATTGACTACACTGAAAATCTACAATTGATAAAGG
AGATAAAAGGAGCTCAGTACGGAGTGCAGCTAGAGAATACGGTGAGAGATCGTTTGGCTG
AGGAGGATAAGTACGAGGAAGAAGAAGAAACTGAGCTCGAGAAGGTCGTTGGCTTTTTCC
AGTCCAAGTACTTCAAAGCTAACTCCGTTATCACTTACCATTTCTCAGCCAAAGATGGCA
TTTGCGAGGTAAGAAAATATCAACTTTCATATATTTTTAGAAAGAAAGTATGTTTAGTTA
TCCATAAACATAAGCATCAAATTCTTGTGGAACAGAGGAACTCTGTTTTTTGCGTTATAC
AGTATAACTTATACTAATGAGAATTTGTTTGAAAGAGTAGATGTTTTAGTTTCTTATTGC
TAGCGTACAAAACAAT
Primers used for PCR 68yp - 8474, show primer details
   
Sequence Name 07-K121888-0022-189-B03C-68yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 68yp: 5'-TCTAAACCCGTAATAGCGGAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K121888-0022-189-B03C-68yp
GATTGGGAGTCAGATAAAAGAAACGAAAAGGGTTTTTAACGTTACTGCTTTAAAGACACT
CTTTATTTCTCTTTTTTTTTTTTTTTTTTTTTGGGGGGCCTTGCCCCCCCCCTTTTTTTT
TTCTTTCCGGTTTTCAAAACCCCTTGGGAAAAAAGGGGGCCCCCCCCATTTTTTGTCCCA
ATTTTGGGCCCCTTTTTTTCCAATTTAAACCCCAACCCCTTTTTTGGGGAAAAAAAAAAT
TTTTCTCCCCCCCCTTTTAAAAAAAAAACCCCCCCCCCCCCCTTTTTTTAGGAAAAAAAG
GGGGGGGGGGAAGGGGGGGTTAACCCAAAACCGGGGCCCCCGGGGGGGGCCCCAAAAAAA
AATGGAAAACCCCCCTTTTGGGGCCAAAAAAATTTAATTCCAAAAAAAAAACCCCCCCCT
TTTAAAACCCAACCTTTAAAGGTTAAAAAAATTTTTTTCCCCCCCCCCCCCCCCGGGGTT
TTTTAAAAAGAGAAAAATTCCCCCTTTCCCATTTCCAATTGGTTCCCCCAAAAACGAAAA
AAAACCCCCCCCCCCCTTTAAAAATTTCCCCCCCCCCCAATCCCAAAAAAAAAAAATAAA
AAGGGGGGCCCCCCCCCCCTGGGCTTTTTTTTTTAAACAAGTTTTCCCCCCAAAAAAAAA
TTTTCCTTTCCAAAAAGTTCCAAAATTCCCCCGGGGCTTTTTTCCAGGGGGGGTTTTTTT
CCCAAAAAATGGGGGGTTCTTTTTTAAAAAAACGGGGAAAAAAAAAGGGGCGGGGTTCCA
AAGGTTCTTTTTCCCCCCAAAAAAACCCTCTCCACCCCCCTTTTTTT
   
Sequence Name 01-K121887-0022-189-B03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K121887-0022-189-B03C-8409
CTCCATCTCCGCTGAAAATGTATAATAGTAAGCAGAGGAACCGGTTCAATTTCGTTGGAC
ACGTACTTAACCAGATTAATTAAGTAAACCGGAGTTTAACCAGTTGAATCAAAGTAAACC
AAAATAAGAAGCCAAACCAAATAATGTATTTATTGAACCACGTAGTCTCCATCTAAACCA
GAGAACCCTAATTCAAATTTTGATTTGAAAACATGGACTAATTAAGATTACCGGAGGCAA
GGGCGTCGAAGAAGTCATCATCGCCGGCGAGTTCTTTTCCGGTTTTGCCTTTCCAGTTAT
CAAGATGTGTTTTAACATCTGAAGGATCTAAGTAAACTCCGATCGCAGTGAACTTCACTT
GAAGAAAGTGGATCTCAATGTCTGTGATCCCTATAACAAGAATATGAACAATCCATATAA
AATTATTGTTACCTGCGATTTTGTTATGTATCGCATTATAAGGTATCAGACATTAAGAAA
GCAAAAAAGAAATAAAAACCTTGGCCCAGAAGAGAGAGTGGCTTGGAAGTGATGATCTGT
GGAGGAAAAGGAACCTCGTGAACCATGACCATCTCTGTTCCCACTGTTTGATAAACAAGA
ACACACAAATCTTAGGAAAAAAACAAAGCATTGAAAAAAAGACAATGAGAATAATTGAAA
CTTGTTAGAACTGAAAATCTTACTTTAGTGGATAAACTTGTAATAAAAAAGAATGCAAAG
AGTGTAAGACTTACTTTCTAATTTATATTATTTTGAATCTGAGAGTGAAGAAATTTATAA
ATGGCTTGGTGTACTATTTTACGATCTTAGAGAAACAATATCGAAATTGTAAATGTGAAT
ATCTCTCTCTATATAATAAGCCAGGGACTGGTGGTAGGTAACATAATTTTGCTAACGTTC
AAAGCTTGTGATCTAAAAGACGACGTATTCTTTTTATGAATTTCATTTTTTGCTACAAAG
CTTGTGATCTCAATTGTTTGT

Last Updated on 06.12.2018 16:11