Sequences from confirmation amplicon


Confirmation sequences for line 189B10 and hit At4g33710 are shown.
Line ID 189B10
Locus At4g33710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16181158
Primers used for PCR 24qc - 8474, show primer details
   
Sequence Name 58-K115305-0022-189-B10C-24qc
GenBank Accession LN509788 [GenBank]
Sequencing primer gene specific primer, 24qc: 5'-ACATTAAATGAGATAAGAACGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K115305-0022-189-B10C-24qc
CATCATCGCCATACATTCACGTGATCTACTAAATATCATAGAAATTAAACTAGCATAAAA
CTGAGAAATTATCTAGAGGATAAAAATAACTTTGAACGGTAGTATATTCTAATGATTTGT
ACATTTTTGTGCTTCAAAGTTTCTCCTTAATAATAATAAAATAAAAGCATACCAACGTAT
ATAGAAAGATATGAAGAAATTGTCTTGGATTTTTAATACAGTAAAAATCATTTCAGTCAA
ATCCTTGTTTTAAATTTATTTACAAAGGGGCATTATCAAGGCTCTCTGACCCTTTCGCCT
TATTGACTTGGTAAAACAGTTGATAATAATAATACAATTACTTACAAGTCGTGTCTTCGT
TGGTAAAAAATATCTCGCAAGTGTTATTAATCGTCCATAAAATAACTCCAACCTAAACTC
TATATAAATCCTCGTTTCTAAGTTCTAACCAATAACACACAACATAGAATAAAAGAGCTT
ACAGACAACTAAGAAAAAAAATAAAAATGAAGATGTTTATCTCTCCTCAAACCCTAGTAC
TATTGGCATTAGCTCTTGTCCTAGCTTTTGCAGTTCCACTGAAAGCCCAAGACCGGCGAC
GTTAGTGTCCCGGACAGGATATATTCAATTGTAAATGGCTACATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGT
   
Sequence Name 23-K115304-0022-189-B10C-8409
GenBank Accession LN496679 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K115304-0022-189-B10C-8409
TTTCATTGAATATATCCTGTCCGGGACACTAACGTCGCCGGTCTTGGGCTTTCAGTGGAA
CTGCAAAAGCTAGGACAAGAGCTAATGCCAATAGTACTAGGGTTTGAGGAGAGATAAACA
TCTTCATTTTTATTTTTTTTCTTAGTTGTCTGTAAGCTCTTTTATTCTATGTTGTGTGTT
ATTGGTTAGAACTTAGAAACGAGGATTTATATAGAGTTTAGGTTGGAGTTATTTTATGGA
CGATTAATAACACTTGCGAGATATTTTTTACCAACGAAGACACGACTTGTAAGTAATTGT
ATTATTATTATCAACTGTTTTACCAAGTCAATAAGGCGAAAGGGTCAGAGAGCCTTGATA
ATGCCCCTTTGTAAATAAATTTAAAACAAGGATTTGACTGAAATGATTTTTACTGTATTA
AAAATCCAAGACAATTTCTTCATATCTTTCTATATACGTTGGTATGCTTTTATTTTATTA
TTATTAAGGAGAAACTTTGAAGCACAAAAATGTACAAATCATTAGAATATACTACCGTTC
AAAGTTATTTTTATCCTCTAGATAATTTCTCAGTTTTATGCTAGTTTAATTTCTATGATA
TTTAGTAGATCACGTGAATGTATGGCGATGATGCTAATTAAATAATGCATTTTATTGTGA
AAACTCGTTCTT

Last Updated on 06.12.2018 16:11