Sequences from confirmation amplicon


Confirmation sequences for line 189E02 and hit At4g17860 are shown.
Line ID 189E02
Locus At4g17860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9925854
Primers used for PCR 12qv - 8474, show primer details
   
Sequence Name 65-K115779-0022-189-E02C-12qv
GenBank Accession LN511465 [GenBank]
Sequencing primer gene specific primer, 12qv: 5'-CTATAAGCAACCTGCATTTCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K115779-0022-189-E02C-12qv
GTGGGTTCATGATCATTAGAAAGATGAGTTTATCTATACACAATTTCATATCAAAATTAT
TTATCAAGTGCTTATTTGTCTCTATCCTAGATGAGACCAAGTGTCGATTTTCAAACCACG
GTATCGACGGAGCCGGAAACATCAGATTTATTCACTGGTAAAGCAGAAGAAAGGTGTCCT
AAAGGACAAGTACCAATTCACATACCGCAAATTAACTATACTAATGACTTTGTCCAACCG
AATAGAAAAAGAAAAAGAAATGGAGAAACGACAATGGCTAAAATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 17-K115778-0022-189-E02C-8409
GenBank Accession LN493994 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115778-0022-189-E02C-8409
TCGGACTGACCATTTACAATTGAAATTTTAGCCATTGTCGTTTCTCCATTTCTTTTTCTT
TTTCTATTCGGTTGGACAAAGTCATTAGTATAGTTAATTTGCGGTATGTGAATTGGTACT
TGTCCTTTAGGACACCTTTCTTCTGCTTTACCAGTGAATAAATCTGATGTTTCCGGCTCC
GTCGATACCGTGGTTTGAAAATCGACACTTGGTCTCATCTAGGATAGAGACAAATAAGCA
CTTGATAAATAATTTTGATATGAAATTGTGTATAGATAAACTCATCTTTCTAATGATCAT
GAACCCACATACCTGGATTTGGTGATCTTTCATCAATGGATGTTGAAATGCAGGTTGCTT
ATAGA

Last Updated on 06.12.2018 16:11