Sequences from confirmation amplicon


Confirmation sequences for line 189F01 and hit At1g01580 are shown.
Line ID 189F01
Locus At1g01580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:211397
Primers used for PCR MJ10 - 8474, show primer details
   
Sequence Name 50-K018511-022-189-F01C-MJ10
GenBank Accession LN507024 [GenBank]
Sequencing primer gene specific primer, MJ10: 5'-TGAAATGCAAGTCAATCAAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K018511-022-189-F01C-MJ10
CTTAACATACCTGAATATAAAGTTATTAGATAGGTGATTGAGAAAGTGTATATAAACAAA
TCACCTTAAGAAATCGGCGGAAGCAGGGCCGTAAGGACCTTCTACAGAAACAGCAAGTCG
GTCGATCTGATCAGAAGAAGAAAGCCTCTGGTGAAGCTTAGTTGACCATTTGCCTTCTTT
CTTGATAACAATACTTAGCTTCTCTGGTTCGAGTTTGCTACTGGAAGTTATCGTAAACGG
ATGCCATTGCAGCTTTGAAATGCTCGGTATGTTCACAAACATTATGCTCGTCGGGCTATA
AACCAACTCTATAAAACAAAAAGAATAGTTTAGAATTAGATCAATATCCAGTAAGGGTGA
ACTTTTCATATACAATTAGCCAATTTGTCCGGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 51-K018511-022-189-F01C-8409
GenBank Accession LN507351 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K018511-022-189-F01C-8409
TCCCGGTCCTGAAGCCATTTACAATTGAATATATCCCGGACAAATTGGCTAATTGTATAT
GAAAAGTTCACCCTTACTGGATATTGATCTAATTCTAAACTATTCTTTTTGTTTTATAGA
GTTGGTTTATAGCCCGACGAGCATAATGTTTGTGAACATACCGAGCATTTCAAAGCTGCA
ATGGCATCCGTTTACGATAACTTCCAGTAGCAAACTCGAACCAGAGAAGCTAAGTATTGT
TATCAAGAAAGAAGGCAAATGGTCAACTAAGCTTCACCAGAGGCTTTCTTCTTCTGATCA
GATCGACCGACTTGCTGTTTCTGTAGAAGGTCCTTACGGCCCTGCTTCCGCCGATTTCTT
AAGGTGATTTGTTTATATACACTTTCTCAATCACCTATCTAATAACTTTATATTCAGATT
TGTTAAGAGTCCTGGAAACTTGATTGACTTCCATTT

Last Updated on 06.12.2018 16:11