Sequences from confirmation amplicon


Confirmation sequences for line 190B01 and hit At2g34020 are shown.
Line ID 190B01
Locus At2g34020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14373145
Primers used for PCR BL76 - 8474, show primer details
   
Sequence Name 21-K105305-0022-190-B01C-BL76
GenBank Accession LN495655 [GenBank]
Sequencing primer gene specific primer, BL76: 5'-TTGTACATCCAATAAATCAGGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K105305-0022-190-B01C-BL76
AGTCAGAGCATAATCCCTGCAGCTTTTTTGTTCTTAGGATCCGCATCTACGCTTGCTTCT
GCAGAGAGCGAGAAACCGCTAAATCGATGAGTCTTCACACAAAAAGAGAAGAGAAGGAAT
CTGCAAGTATTCTTAACGTACCAACAACACTTTTTAGTATCTTCATCCTGTAAAATCCTC
TTCTTGGATTCTTTGTGTCCTGTATATTAACAGATTATATTCATTGAAATTAGAACTTGT
TACTAATCTCTTATACTAAGAGATAGGAACAAAATAATCATTTCTTACAGAGGATGTTCT
TTTGATAGATCCTCTTCGGATTGATGGATCTGAATTAGGACTAGTCAAACCAAAGATAAC
ACAAGCTCCCCATTGCATTGTCAGAGCAAAGATTGTGTGTCCAATAGTAACTCCAACGTT
GTTTCCAACAATCATTAAAGCACCCTCGTGGGTCGCTGCTAGTCCGGGAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K105306-0022-190-B01C-8409
GenBank Accession LN488347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K105306-0022-190-B01C-8409
TTTCCCGGACAGCAGCGACCCCGAGGGTGCTTTAATGATTGTTGGAAACAACGTTGGAGT
TACTATTGGACACACAATCTTTGCTCTGACAATGCAATGGGGAGCTTGTGTTATCTTTGG
TTTGACTAGTCCTAATTCAGATCCATCAATCCGAAGAGGATCTATCAAAAGAACATCCTC
TGTAAGAAATGATTATTTTGTTCCTATCTCTTAGTATAAGAGATTAGTAACAAGTTCTAA
TTTCAATGAATATAATCTGTTAATATACAGGACACAAAGAATCCAAGAAGAGGATTTTAC
AGGATGAAGATACTAAAAAGTGTTGTTGGTACGTTAAGAATACTTGCAGATTCCTTCTCT
TCTCTTTTTGTGTGAAGACTCATCGATTTAGCGGTTTCTCGCTCTCTGCAGAAGCAAGCG
TAGATGCGGATCCTAAGAACAAAAAAGCTGCAGGGATTATGCTCTTGACTCTAGTTCCAT
TTCTCTTAGTGACATTGCCTGATTTATTGGATGTACA

Last Updated on 06.12.2018 16:11