Sequences from confirmation amplicon


Confirmation sequences for line 190G09 and hit At1g63090 are shown.
Line ID 190G09
Locus At1g63090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23391319
Primers used for PCR SR08 - 8474, show primer details
   
Sequence Name 76-K103584-0022-190-G09C-SR08
GenBank Accession LN513384 [GenBank]
Sequencing primer gene specific primer, SR08: 5'-GTGGTTTCAAGTTGATGGAGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K103584-0022-190-G09C-SR08
TACTTCAGGGCCTTTAGCCAGGCAAACCAGGCAAGCGGTTTGGTTGGAAGGTTGTTGACA
CCGAACAGGTTCATGGCTGGAACATTAAACCTGTTCGTTTTCAACTCTCGACTGAAGATG
GTCAACATTCTTCATCTCAATGTATGTTAACCGAAGCAGGAAACTGGAGTCATTACCATG
CTGGAGACTTTGTGGTCGGTAAATCGAAAAGTTCATCGACGAAGATTAAGTTCTCGATGA
CTCAGATTGATTGCACGCATACTAAAGGCGGGTTATGTGTAGATTCTGTAGTTGTGTATA
TATTCAATTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TG
   
Sequence Name 82-K103585-0022-190-G09C-8409
GenBank Accession LN514182 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K103585-0022-190-G09C-8409
AGCCATTTATAATTGAAATATACACAACTACAGAATCTACACATAACCCGCCTTTAGTAT
GCGTGCAATCAATCTGAGTCATCGAGAACTTAATCTTCGTCGATGAACTTTTCGATTTAC
CGACCACAAAGTCTCCAGCATGGTAATGACTCCAGTTTCCTGCTTCGGTTAACATACATT
GAGATGAAGAATGTTGACCATCTTCAGTCGAGAGTTGAAAACGAACAGGTTTAATGTTCC
AGCCATGAACCTGTTCGGTGTCAACAACCTTCCAACCAAACCGCTTGCCTGGTTTGCCTA
GCTGAAGCCTGAAGTAAACACTGTAGGTTCCAGCTGGAAATGGGAAATCAATTTCTCCAT
CACTTGGAAAACC

Last Updated on 06.12.2018 16:11