Sequences from confirmation amplicon


Confirmation sequences for line 191A08 and hit At1g21600 are shown.
Line ID 191A08
Locus At1g21600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7573746
Primers used for PCR BL77 - 8474, show primer details
   
Sequence Name 31-K105138-0022-191-A08C-BL77
GenBank Accession LN500159 [GenBank]
Sequencing primer gene specific primer, BL77: 5'-TAGAGTCACTGGGAATTCACACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K105138-0022-191-A08C-BL77
GATCAGGAGGTTTTCTGATAAGAAATCACAGTCCATTAAGCTGAGTTTATGAAACTCATC
TTCGTTTATCCGATAATCAACCACCATATCCGAATCCCATCCTTGTGTCGATATAAAACT
CTTACTCTGAACAAAAGCGATATCTCCATCACCACCACCGCCTGCAGCGGCTATCGCGGC
GGCCAAGGGGTCATACTCGACATCATAGTCCTTCTTGTTGTCAACTTCCTCTACCTCCTC
ATCATCCATATCATACTCGTCCGCTCCACCTCCATCAGCATCGACGTCATCTACTTTAAC
GACTATACCACGGCCGCGTCGGGATTTCAGTGTTAAAGGCGCGAAAAGACCGTCCGTTCT
GAATGACGCAAATAGCCGCTGAGAGCCGGAGGGATAAGGTGGCTTCGAGGTGAATGAGAG
GGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCC
   
Sequence Name 15-K105139-0022-191-A08C-8409
GenBank Accession LN492966 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K105139-0022-191-A08C-8409
GCCATTTACAATTGAATATCCCTCTCATTCACCTCGAAGCCACCTTATCCCTCCGGCTCT
CAGCGGCTATTTGCGTCATTCAGAACGGACGGTCTTTTCGCGCCTTTAACACTGAAATCC
CGACGCGGCCGTGGTATAGTCGTTAAAGTAGATGACGTCGATGCTGATGGAGGTGGAGCG
GACGAGTATGATATGGATGATGAGGAGGTAGAGGAAGTTGACAACAAGAAGGACTATGAT
GTCGAGTATGACCCCTTGGCCGCCGCGATAGCCGCTGCAGGCGGTGGTGGTGATGGAGAT
ATCGCTTTTGTTCAGAGTAAGAGTTTTATATCGACACAAGGATGGGATTCGGATATGGTG
GTTGATTATCGGATAAACGAAGATGAGTTTCATAAACTCAGCTTAATGGACTGTGATTTC
TTTATCAGAAAACCTCCTGATCCAGATAATGATGTTTATGATTTCAGAGAGGTGTGTGAA
TTCCCAGTGGACTCT

Last Updated on 06.12.2018 16:11