Sequences from confirmation amplicon


Confirmation sequences for line 191H05 and hit At2g01660 are shown.
Line ID 191H05
Locus At2g01660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:292084
Primers used for PCR EP38 - 8474, show primer details
   
Sequence Name 22-K103231-0022-191-H05C-8409
GenBank Accession LN496073 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K103231-0022-191-H05C-8409
GTAGACGGTGGTGGTTCGACGTTGTGGACCACCGGTTGGTTATAACTCGGACGAGATGAC
TCGTAGAGACTCGGTGGTGGGTTCTTTAGCGGCGAGTAGCGGTGGTTCATACAGAGTAGG
AGTGTCGGGGGAACTGCAAGGTGTGGCGCAGTGCACCGGAGATCTTAGTGCGACTGAGTG
TCAAGACTGTTTGATGGAAGCGATCGGACGGCTGAGAACGGATTGCGGAGGAGCTGCTTG
GGGTGACGTGTACTTAGCAAAGTGCTACGCGCGTTACTCTGCGCGTGGAGGTCACTCTAG
AGCCAACGGCTATGGTAATGTCTTCTTTGCTCCGTTGCTTTTTATTTCTTTTATAAAGAT
TTTTATTTGATTTTAAAGTTTTTAATTAAGGGAAAAACCCGAAAAAATCTCCGGCTAATT
TTGNTTTGTCTG
   
Sequence Name 70-K103232-0022-191-H05C-EP38
GenBank Accession LN512368 [GenBank]
Sequencing primer gene specific primer, EP38: 5'-CAGACAAACTAAAATTAGCCGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K103232-0022-191-H05C-EP38
CTTATTAAACTTTAAATCAAATAAAAATCTTTATAAAAGAAATAAAAAGCAACGGAGCAA
AGAAGACATTACCATAGCCGTTGGCTCTAGAGTGACCTCCACGCGCAGAGTAACGCGCGT
AGCACTTTGCTAAGTACACGTCACCCCAAGCAGCTCCTCCGCAATCCGTTCTCAGCCGTC
CGATCGCTTCCATCAAACAGTCTTGACACTCAGTCGCACTAAGATCTCCGGTGCACTGCG
CCACACCTTGCAGTTCCCCCGACACTCCTACTCTGTATGAACCACCGCTACTCGCCGCTA
AAGAACCCACCACCGAGTCTCTACGAGTCATCTCGTCCGAGTTATAACCAACCGGTGGTC
CACAACGTCGAACCACCACCGTCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11