Sequences from confirmation amplicon


Confirmation sequences for line 192A11 and hit At5g47340 are shown.
Line ID 192A11
Locus At5g47340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19210269
Primers used for PCR 52mm - 8474, show primer details
   
Sequence Name 33-K114664-0022-192-A11C-52mm
GenBank Accession LN501099 [GenBank]
Sequencing primer gene specific primer, 52mm: 5'-TTTGGAGTAATAACCTCATCGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K114664-0022-192-A11C-52mm
TCAAAATGGTTTTGATGAACCCAAACCTTGACAAAAACCGGGTTGTGTAAACGGGTAAAA
CGGTCTTTGAAATTTGGGTTTCTTTGGTTGGGTATCCCGTTGTTAACCTAAGGTAAATAC
TTAAATCTTTCCAAGTACGGTTTCAAATCCGGAGAATAAATAAAAAAAAACCAAAGATTT
TGTTAAAACTTTATGTTGTTGTTTTGACCACCAACCACTTATTTGTTGATTTTGCAAGTT
TTCTTATAAACAAATGGTGAAGGGACTTACTTTAGGGATTTTGTAGTACCCCCTAGAACC
AAAATAATCCTGCAAAGTCATTGAAAATTTTTTATAATTTTCAAATTGAGTCTTTAACCA
GGATATATTTGGAGAAAAAAATATTTTTCCTTGAAGGGTCCCCCTATAAAAAGCCCCCTT
GATCAACTCATTAAATTTCTAACATGCGGGATCGAACTTTACCTTAAAAAAAAAAAACCA
AAACAAACCATTCGTTGTTGTTCCCTTGATGGATAAAAATTAAATTACATGTCCGGGAAA
TCTACTTGGATCACCAATGAGTATAATGGTCAATATGGAAAAAAAAAAAAATTAATTCCC
AATTTTTTTTCATTTCAAAAATGTAAATTTCC
   
Sequence Name 04-K114663-0022-192-A11C-8409
GenBank Accession LN488019 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114663-0022-192-A11C-8409
TTTATTTTATCAATCAAGTAACAACAACGATTGTTTTGTTTTGTTTCCTTTTTTATAAGT
TAACGTCCGATCCAGCATGTAAGAAATTTCATGAGTTGATCAAGGGAGCTCTCTATAGCG
AGACCATTCAAGTATATATATCTCTTTCTCTATATATATCATGATTAAAGACTCAATTAG
AAAATAATAAAAAATCTTCAATGACTCTGCAGGATCATCTTGCTCCTAGTGGTTACTACA
AAATCCCTAATGTAAGTCCATTCACCATTTGTTTATAAGAAAACTTGCAAAATCAACAAA
TAAGTGATTGATGATCAAAACAACAACATAAAGTTTTAACAAAATCTTTGTTTTTCTTTT
ATTTATTCTCAGGATATGAAACAGTACTTGGAAAGATCTAAGTATCTACCTAAGCTTAAC
AACGAGATACCAAACCAAAGAAACCAAACTTACAAAGACCGTTTCACCAGTTTACACAAC
CTGGTTCTTGTCAAGGTTTGGTCTCATCAAAACCATCTAGACTTTTCAACTATCTNCTCA
TTCCTTTCTTGATGACTCTNTTTTTTTTTTTTACAGTTTCAGGACAATAAGTTTATAACT
CCAAAA

Last Updated on 06.12.2018 16:11