Sequences from confirmation amplicon


Confirmation sequences for line 192B04 and hit At2g44800 are shown.
Line ID 192B04
Locus At2g44800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18467512
Primers used for PCR ZZ94 - 8474, show primer details
   
Sequence Name 21-K023619-022-192-B04C-8409
GenBank Accession LN495495 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K023619-022-192-B04C-8409
TAGATTTCCCGGACATGAAGCCACAAAATGCATGCATGCAATGTTTTCTCTACTCTGTAC
GACTGTACAGAACTAAGAGATAAAGGCCCTAACTTTTACTTCCCTACAGGTTATAAACCA
TGGAATACCATCATCAGTGGTTAACGATGCCTTAGATGCAGCAACACAGTTCTTCGACTT
ACCTGTGGAAGAGAAGATGCTTCTGGTGTCTGCAAACGTTCATGAGCCAGTCAGGTATGG
CACAAGCCTTAACCATTCAACAGACAGAGTTCACTACTGGAGAGATTTCATCAAGCATTA
CTCTCATCCACTCTCAAAGTGGATCGATATGTGGCCATCAAATCCTCCATGCTACAAGT
   
Sequence Name 22-K023619-022-192-B04C-ZZ94
GenBank Accession LN495943 [GenBank]
Sequencing primer gene specific primer, ZZ94: 5'-TTACTTGTAGCATGGAGGATTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K023619-022-192-B04C-ZZ94
TGGCCACATATCGATCCACTTTGAGAGTGGATGAGAGTAATGCTTGATGAAATCTCTCCA
GTAGTGAACTCTGTCTGTTGAATGGTTAAGGCTTGTGCCATACCTGACTGGCTCATGAAC
GTTTGCAGACACCAGAAGCATCTTCTCTTCCACAGGTAAGTCGAAGAACTGTGTTGCTGC
ATCTAAGGCATCGTTAACCACTGATGATGGTATTCCATGGTTTATAACCTGTAGGGAAGT
AAAAGTTAGGGCCTTTATCTCTTAGTTCTGTACAGTCGTACAGAGTAGAGAAAACATTGC
ATGCATGCATTTTGTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTC

Last Updated on 06.12.2018 16:11