Sequences from confirmation amplicon


Confirmation sequences for line 192B12 and hit At5g43760 are shown.
Line ID 192B12
Locus At5g43760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17586663
Primers used for PCR AY40 - 8474, show primer details
   
Sequence Name 59-K021739-022-192-B12C-8409
GenBank Accession LN509868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K021739-022-192-B12C-8409
TTGATCTCGCTAAACAGATGCTTCAGGTAAAATATCTTATTATTCGGTTTGGTTTAATCC
GGTTTGGTCTTTTAGACTGTTATGAGTCGTCGTGATTCGTGGAAGAATAAAATCTAGGAC
TTTTTCTTATTCGACTTTAAAAATTTGAAAGGGCTAATGACAGCTCATGATTTTAGAAAT
TATTTAGGGGAATTCAATAGAACTACCAGCTAGAATTTGCCTTTCAAAATTTAATTATTT
GCGTCTTGGTGTCACTTAATAAAGCATTTAGTACACTTACCAGCTAGATTTTTTGATTAA
TTGACTTAGCTTTCATGTCATTAATACGTGCTATAATATTTCTATGACTTATTTGATCAA
AATGTAGTCACTGGATTGGTTTTTAGTTGCCGACTTAATATGGATGCAACTTTATTCTAT
ATGAGTTGTATGAAAGCATAAAGGCCAAATTTACGTGTAAAAGTCACGTGTCTATGAAAA
TTCTGACAGTGACAGGCTTTGTAGTCTTTAGGTTGGTGGTAGTTGTGAAAATGGAAACCC
CATAAT
   
Sequence Name 60-K021739-022-192-B12C-AY40
GenBank Accession LN510135 [GenBank]
Sequencing primer gene specific primer, AY40: 5'-CATTATGGTTTTTCCATTTTCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K021739-022-192-B12C-AY40
TCAGAATTTTCATAGACACGTGACTTTTACACGTAAATTTGGCCTTTATGCTTTCATACA
ACTCATATAGAATAAAGTTGCATCCATATTAAGTCGGCAACTAAAAACCAATCCAGTGAC
TACATTTTGATCAAATAAGTCATAGAAATATTATAGCACGTATTAATGACATGAAAGCTA
AGTCAATTAATCAAAAAATCTAGCTGGTAAGTGTACTAAATGCTTTATTAAGTGACACCA
AGACGCAAATAATTAAATTTTGAAAGGCAAATTCTAGCTGGTAGTTCTATTGAATTCCCC
TAAATAATTTCTAAAATCATGAGCTGTCATTAGCCCTTTCAAATTTTTAAAGTCGAATAA
GAAAAAGTCCTAGATTTTATTCTTCCACGAATCACGACGACTCATAACAGTCTAAAAGAC
CAAACCGGATTAAACCAAACCGAATAATAAGATATTTTACCTGAAGCATCTGTTTAGCGA
GATCAATGGAGATAAGGCCAGCACTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11