Sequences from confirmation amplicon


Confirmation sequences for line 193D07 and hit At2g29670 are shown.
Line ID 193D07
Locus At2g29670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12682470
Primers used for PCR KA21 - 8474, show primer details
   
Sequence Name 23-K105305-0022-193-D07C-KA21
GenBank Accession LN496551 [GenBank]
Sequencing primer gene specific primer, KA21: 5'-CCGAAATTGCATTACGATTTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105305-0022-193-D07C-KA21
GTGCAACCAATAAAAAAGAAAAGTGTACAGTAAACACACTTGGTTCCACGACATAGGCCC
AACCGAAGATTCATAAGTTATGAGTCATAACTCATGAAGGCGGTGAGATCAGGTTCGATC
TCATGCGCTATAATCACGTGCCTCAACTACCACCAAACCAAGATATTTTCTTCCTTTTCT
CTAGACTGATCGCAACCATAAGTATTATATAACTCTTTTACCCACCGAGCCTTGGTGACA
AACATGCATATACATAATACATTAATGGTATGCACATATGTGTATATTCATTAGTTGTAC
TTATTGTATACAATATTTTATATAGAGAGACTTTGATGGTGCACATTCTTATCTAAATGG
TGTCTTCATGAGACTCGTCATCAAGAGGGAAACACGTGTCATCACCGCCGGTATTCCACA
AGAAATTGGCGTAATTAGCGGCGTAGTAAGAGTTGGTCGGATCGGCATCAATTGCTTCTA
AGAAAGTCTCCTCAGCCGCCCAAAGATCGTCCCGGACAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 07-K105306-0022-193-D07C-8409
GenBank Accession LN489265 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105306-0022-193-D07C-8409
GCCATTTACAATTGAATATATCCTGTCCGGGACGATCTTTGGGCGGCTGAGGAGACTTTC
TTAGAAGCAATTGATGCCGATCCGACCAACTCTTACTACGCCGCTAATTACGCCAATTTC
TTGTGGAATACCGGCGGTGATGACACGTGTTTCCCTCTTGATGACGAGTCTCATGAAGAC
ACCATTTAGATAAGAATGTGCACCATCAAAGTCTCTCTATATAAAATATTGTATACAATA
AGTACAACTAATGAATATACACATATGTGCATACCATTAATGTATTATGTATATGCATGT
TTGTCACCAAGGCTCGGTGGGTAAAAGAGTTATATAATACTTATGGTTGCGATCAGTCTA
GAGAAAAGGAAGAAAATATCTTGGTTTGGTGGTAGTTGAGGCACGTGATTATAGCGCATG
AGATCGAACCTGATCTCACCGCCTTCATGAGTTATGACTCATAACTTATGAATCTTCGGT
TGGGCCTATGTCGTGGAACCAAGTGTGTTTACTGTACACTTTTCTTTTTTATTGGTTTGC
ACATAGAGTACTACGTACAAAACTAGTCAAAACACTAAAATCGTATGCAAATTTCGG
Primers used for PCR AJ20 - 8474, show primer details
   
Sequence Name 03-K105564-0022-193-D07C-AJ20
GenBank Accession LN487485 [GenBank]
Sequencing primer gene specific primer, AJ20: 5'-CCGAAATTGCATTACGATTTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105564-0022-193-D07C-AJ20
CTATGTGCAACCAATAAAAAAGAAAAGTGTACAGTAAACACACTTGGTTCCACGACATAG
GCCCAACCGAAGATTCATAAGTTATGAGTCATAACTCATGAAGGCGGTGAGATCAGGTTC
GATCTCATGCGCTATAATCACGTGCCTCAACTACCACCAAACCAAGATATTTTCTTCCTT
TTCTCTAGACTGATCGCAACCATAAGTATTATATAACTCTTTTACCCACCGAGCCTTGGT
GACAAACATGCATATACATAATACATTAATGGTATGCACATATGTGTATATTCATTAGTT
GTACTTATTGTATACAATATTTTATATAGAGAGACTTTGATGGTGCACATTCTTATCTAA
ATGGTGTCTTCATGAGACTCGTCATCAAGAGGGAAACACGTGTCATCACCGCCGGTATTC
CACAAGAAATTGGCGTAATTAGCGGCGTAGTAAGAGTTGGTCGGATCGGCATCAATTGCT
TCTAAGAAAGTCTCCTCAGCCGCCCAAAGATCGTCCCGGACAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 51-K105565w-0022-193-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K105565w-0022-193-D07C-8409
GCCATTTACATTGAATATATCCTGTCCGGGACGATCTTTGGGCGGCTGAGGAGACTTTCT
TAGAAGCAATTGATGCCGATCCGACCAACTCTTACTACGCCGCTAATTACGCCAATTTCT
TGTGGAATACCGGCGGTGATGACACGTGTTTCCCTCTTGATGACGAGTCTCATGAAGACA
CCATTTAGATAAGAATGTGCACCATCAAAGTCTCTCTATATAAAATATTGTATACAATAA
GTACAACTAATGAATATACACATATGTGCATACCATTAATGTATTATGTATATGCATGTT
TGTCACCAAGGCTCGGTGGGTAAAAGAGTTATATAATACTTATGGTTGCGATCAGTCTAG
AGAAAAGGAAGAAAATATCTTGGTTTGGTGGTAGTTGAGGCACGTGATTATAGCGCATGA
GATCGAACCTGATCTCACCGCCTTCATGAGTTATGACTCATAACTTATGAATCTTCGGTT
GGGCCTATGTCGTGGAACCAAGTGTGTTTACTGTACACTTTTCTTTTTTATTGGTTTGCA
CATAGAGTACTACGTACAAAACTAGTCAAAACACTAAAATCG
Primers used for PCR 46xd - 8474, show primer details
   
Sequence Name 27-K118953-0022-193-D07C-46xd
GenBank Accession LN498617 [GenBank]
Sequencing primer gene specific primer, 46xd: 5'-GTCATAATAATCGCATCAGCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118953-0022-193-D07C-46xd
ATTTCTTTTCTCTTTGAGAAAACGACATTACACAAATATCTAACAGAGAATAAATAAAGA
AAATTATAATGTAACTCTCAGTCAGTGTTAGGGATAAGTTCTAGCCAATGAGCTTTGAGT
CTTTGAGGGTTTTAGTGATAGTGGAGTTTACGGTTGTGTTTTAAAGACTCCCCACCATAA
CCCAACAACAAAAGTCGTTACATCACAATTTGTCTTCTAGATTTTATCTCCCACCCAAAT
TAATTAACCCATGTTTTTGTCATTAGCTTTATACATGTGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTCTTCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGAGTTATA
   
Sequence Name 11-K118952-0022-193-D07C-8409
GenBank Accession LN491345 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118952-0022-193-D07C-8409
ATTGGTAATTACTCTTTCTTTTTCTCCACATGTATAAAGCTAATGACAAAAACATGGGTT
AATTAATTTGGGTGGGAGATAAAATCTAGAAGACAAATTGTGATGTAACGACTTTTGTTG
TTGGGTTATGGTGGGGAGTCTTTAAAACACAACCGTAAACTCCACTATCACTAAAACCCT
CAAAGACTCAAAGCTCATTGGCTAGAACTTATCCCTAACACTGACTGAGAGTTACATTAT
AATTTTCTTTATTTATTCTCTGTTAGATATTTGTGTAATGTCGTTTTTCCTCAAAGAGGA
AAAGAAATTGGTTGGTGTATATTCTGCTGATGCGATTATTATGAC

Last Updated on 06.12.2018 16:11