Sequences from confirmation amplicon


Confirmation sequences for line 193E06 and hit At2g18800 are shown.
Line ID 193E06
Locus At2g18800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8146157
Primers used for PCR 13ru - 8474, show primer details
   
Sequence Name 47-K116229-0022-193-E06C-13ru
GenBank Accession LN506305 [GenBank]
Sequencing primer gene specific primer, 13ru: 5'-AACCCATGAGAATGTATGGAAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116229-0022-193-E06C-13ru
CTTTTTTTTTTTTCGAGCAGTACTAGCGAGTGGTTTTCCGCAGAGAGGGATGGATTTCTT
CAAGCAAGAAAGTTCTTAGATGGGTTTCAGAGGAAGTTCATGGTTTACAATTATTGTAAG
GACAAGAAGCGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTCAATTC
AAAAATGT
   
Sequence Name 07-K116228-0022-193-E06C-8409
GenBank Accession LN489427 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116228-0022-193-E06C-8409
ATTCTAAGAACTTTTCTGGCTTCGAAGAATCCCATCCCTCTTCTGCGAAAACCACTCGCT
AGTACTCGCTTCGAAGAAGAAGAAGTCGAGTTCACCGGGGCTACAAGGAGAAGTAGTTGT
GGTTCCATTAACGATGGACCAAACACAAGCATTCTCGGAGTTGTAGTTCATGAAAGAGGC
TACGAATGGTCCCTGTGACCAGTTTGTCTTTACCAGCCCTCCCCTCGTGGCCCAATCGTC
TGCGTTCCATAGACTTCCATAC

Last Updated on 06.12.2018 16:11