Sequences from confirmation amplicon


Confirmation sequences for line 193G07 and hit At3g22275 are shown.
Line ID 193G07
Locus At3g22275 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7879563
Primers used for PCR 02pt - 8474, show primer details
   
Sequence Name 27-K115331-0022-193-G07C-02pt
GenBank Accession LN498524 [GenBank]
Sequencing primer gene specific primer, 02pt: 5'-GTTTCCCTCGTATTGAAACATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115331-0022-193-G07C-02pt
CTATTTTTTTTCCCCAAAAAAAAGTCATAGCACGTTCTTATATATACCTTACATGCAACC
ATTTCAATATTGATTACTTTGATACGAAAATCGACCAAAGTAAGAATATTTACCTAGAGA
GGATCATGAAGGGTTGCAGCTTAAATCTTCACCTATCTCCAATGGCCTCTACGCTTCAAT
CTTGTCATCAAGATTCTACGTGAGTTTTATCATCCAAATCACTCTACTTTCATATTTAAA
CCTCTTAAATCGTTTTGAAAATGAATTTTTTTCATATAGAGTTAATGATCGTTCTTCAAC
CATAAGATCTAAGGAAACCATAAAATGAATTGGATCGTTATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCATTATA
   
Sequence Name 03-K115330-0022-193-G07C-8409
GenBank Accession LN487612 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115330-0022-193-G07C-8409
TCGGACTGAGCCATTTCATTGAATAACGATCCATTCATTTTATGGTTTCCTTAGATCTTA
TGGTTGAAGAACGATCATTAACTCTATATGAAAAAAATTCATTTTCAAAACGATTTAAGA
GGTTTAAATATGAAAGTAGAGTGATTTGGATGATAAAACTCACGTAGAATCTTGATGACA
AGATTGAAGCGTAGAGGCCATTGGAGATAGGTGAAGATCTAAGCTGCAACCCTTCATGAT
CCTCTCTAGGTAAATATTCTTACTTTGGTCGATTTTCGTATCAAAGTAATCAATATTGAA
ATGGTTGCATGTAAGGTATATATAAGAACGTGCTATGACTTTTTTTTTGGTGGAAAAAAA
ATTAGATATCTGCAAATTTGGTCACGTGCTTCAGGTTTCAATACGAGGGAAACAT

Last Updated on 06.12.2018 16:11